仲恺农业技术学院学报 第16卷 究和解决的问题.以原生质体植株再生技术为基础,利用体细胞杂交技术转移胞质雄性不 育基因、多基因和基因组以及某些野生抗性性状等方面,将是未来研究的主流有理由相 信,原生质体培养和体细胞杂交技术与遗传转化技术相辅相成,与常规育种技术结合起来, 将为植物基因组之间的结合和相互作用以及新品种的培育提供了无限的可能性 参考文献 [1]许智宏,卫志明.植物原生质体培养和遗传操作[M].上海:上海科学技术出版,1997.2030 [2 SHEPARD J F. Cultivar dependent cultural refinements in potato protoplast regeneration J]. Pant Science Letters 98226:127132 [3 FU MURA T. Regeneration of rice plant from protoplant[J]. Plant Tissue culture letters, 1985, 2: 7475 [4 SHLLrIO R D, PASZKOOOSKI J, ROIRYKUS L. Agarose plating and a bead type culture technique enable and stimulate development of protoplast derived colonies in a number of plant species[J]. Pant Cell Report, 1983, 2 [5MIISUGU H, HIIOMI M, FUMINORI K Regeneration of flowering plant. fiom difficile fily protoplasts by means of a nurse culture[J]. Hanta, 2002, 215(5): 88-884 [6]黄百渠.植物体细胞遗传学简明教程[M.长:东北师范大学出版社,1991.9596 7]刘选明,官春云.油菜原生质体培养与融合技术的研究进展[J].湖南农业大学学报,1997,3(5):483 [8] KYOAKA J, O100 E, SHIMAMOTO K Plant regeneration from protoplasts of indica rice: genotypic differences in culture response[J]. TAG, 1988,76: 887890 [9 CORAL G Electrofusion of Saccharomyces cerevisiae Auxotrophic Mutants of ldentical Mating Type Using a Labor ratory- Made System[J]. Turkish Journal of Biology 2003, 27(1): 1-5 [10]汪和睦,谢廷栋.细胞电穿孔、电融合、电刺激一原理技术及应用[M].天津:天津科学技术出版社 [11 MATIHEU W M, PURE KJ. Tetraploid potato hybrids through protoplast fusions and analysis of their pe mance in the field[J]. Theor Appl Genet, 1992, 83: 807812 12 ZHOU Arfen, CHEN Xiurling, XIA Guang min, ef al. UV-fusion between potopasts of commn wheat and nalda willasa[] Journal of Plant Physiology and Molecular Biology 2002, 28(4): 305-310 [13]向凤宁,夏光敏,陈惠民小麦与燕麦不对称体细胞杂交的研究[]中国科学C辑),2002,32(4):299 [14]邓秀新,章文才.柑桔原生质体培养与融合研究[].自然科学进展,1995,5(1):3541. [15」肖尊安,韩碧文.猕猴桃属种间体细胞杂种口].植物学报,1997,39:1107 [16]辛化伟.水稻与大黍不对称体细胞杂交再生植株[].植物学报,1997,39(8):717824. [17 WUBRANDIJ, VOS J GM, KOORNNEEF E. Transfer of regeneration capacity from lycopersicon peruvianum to L. esculentum by protoplast fusion[ J]. Pant Cell Tiss Org Cult, 1988, 12: 19]196 [18 SUNDBER E, GIME IUS KA. Method for production of interspecific hybrids within B. raxsiceae via somatic hy bridization using resynthes is of B. rassica napus as a mdel [J]. Plant Sci, 1986, 43: 155 162 [19 CHUONG P V, BEVERSDORF WD, ROWEL A D, et al. The use of haploid protoplast fusion to combine cyto plasmic atrazine and cytoplasmic male sterility in B. rassica napus]. Pant Cell Tissue and Organ Cultu 1988,12:18}-184 201994-2008ChinaAcademicournalElectronicPublishinghOuse.Allrightsreservedhttp://nwy.cnki.net究和解决的问题. 以原生质体植株再生技术为基础 ,利用体细胞杂交技术转移胞质雄性不 育基因、多基因和基因组以及某些野生抗性性状等方面 ,将是未来研究的主流. 有理由相 信 ,原生质体培养和体细胞杂交技术与遗传转化技术相辅相成 ,与常规育种技术结合起来 , 将为植物基因组之间的结合和相互作用以及新品种的培育提供了无限的可能性. 参考文献 : [1 ] 许智宏 ,卫志明. 植物原生质体培养和遗传操作[M]. 上海 :上海科学技术出版 ,1997. 20230. [ 2 ] SHEPARD J F. Cultivar dependent cultural refinements in potato protoplast regeneration[J ]. Plant Science Letters , 1982 ,26 :1272132. [3 ] FUJ IMURA T. Regeneration of rice plant from protoplant[J ]. Plant Tissue culture letters ,1985 ,2 :74275. [4 ] SHILLITO R D ,PASZKOOOSKI J ,POTRYKUS I. Agarose plating and a bead type culture technique enable and stimulate development of protoplast2derived colonies in a number of plant species[J ]. Plant Cell Report ,1983 ,2 : 2442247. [5 ] MITSUGU H , HITOMI M , FUMINORI K. Regeneration of flowering plants from difficile lily protoplasts by means of a nurse culture[J ]. Planta , 2002 , 215(5) :8802884. [6 ] 黄百渠. 植物体细胞遗传学简明教程[M]. 长春 :东北师范大学出版社 ,1991. 95296. [7 ] 刘选明 ,官春云. 油菜原生质体培养与融合技术的研究进展[J ]. 湖南农业大学学报 ,1997 ,3 (5) :4832 492. [8 ] KYOZUKA J ,OTOO E ,SHIMAMOTO K. Plant regeneration from protoplasts of indica rice :genotypic differences in culture response[J ]. TAG,1988 ,76 :8872890. [9 ] CORAL G. Electrofusion of Saccharonmyces cerevisiae Auxotrophic Mutants of Identical Mating Type Using a Labo2 ratory2Made System[J ]. Turkish Journal of Biology ,2003 ,27(1) :125. [10 ] 汪和睦 ,谢廷栋. 细胞电穿孔、电融合、电刺激 —原理技术及应用[M]. 天津 :天津科学技术出版社 , 2000. [11 ] MATTHEIJ W M ,PUITE K J . Tetraploid potato hybrids through protoplast fusions and analysis of their perfor2 mance in the field[J ]. Theor Appl Genet ,1992 ,83 :8072812. [12 ] ZHOU Ai2fen ,CHEN Xiu2ling ,XIA Guang2min , et al. UV2fusion between protopasts of common wheat and Hay2 naldia villosa[J ].Journal of Plant Physiology and Molecular Biology ,2002 ,28(4) :3052310. [13 ] 向凤宁 ,夏光敏 ,陈惠民. 小麦与燕麦不对称体细胞杂交的研究[J ]. 中国科学(C 辑) ,2002 ,32 (4) :2992 305. [14 ] 邓秀新 ,章文才. 柑桔原生质体培养与融合研究[J ]. 自然科学进展 ,1995 ,5(1) :35241. [15 ] 肖尊安 ,韩碧文. 猕猴桃属种间体细胞杂种[J ]. 植物学报 ,1997 ,39 :111021117. [16 ] 辛化伟. 水稻与大黍不对称体细胞杂交再生植株[J ]. 植物学报 ,1997 ,39(8) :7172824. [17 ] WIJBRANDI J ,VOS J G M , KOORNNEEF E. Transfer of regeneration capacity from lycopersicon peruvianum to L . esculentum by protoplast fusion[J ]. Plant Cell Tiss Org Cult ,1988 ,12 :1932196. [18 ] SUNDBER E ,GIMELIUS KA. Method for production of interspecific hybrids within B . rassiceae via somatic hy2 bridization using resynthes is of B . rassica napus as a model[J ]. Plant Sci ,1986 ,43 :1552162. [19 ] CHUONG P V ,BEVERSDORF W D ,POWELL A D ,et al. The use of haploid protoplast fusion to combine cyto2 plasmic atrazine and cytoplasmic male sterility in B . rassica napus[J ]. Plant Cell Tissue and Organ Culture , 1988 ,12 :1812184. 07 仲恺农业技术学院学报 第 16 卷 © 1994-2008 China Academic Journal Electronic Publishing House. 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