Biochemical/Immunological TWo limitations Molecule must be assayable: most applicable if know product and function Measurements on isolated molecules may not accurately reflect function in intact bacterium Prove function in vivo, have to take either genetic or immunological approach BIOL 533 Lecture threeBIOL 533 10 Lecture Three Biochemical/Immunological • Two limitations: – Molecule must be assayable; most applicable if know product and function – Measurements on isolated molecules may not accurately reflect function in intact bacterium • Prove function in vivo; have to take either genetic or immunological approach