第5期 吕乐等：乳酸菌USTB08的高效培养和生产乳酸 ·607· Process Biochem,2006,41(1):120 (熊晓辉，于修鉴，熊强，等.乳酸菌发酵剂高密度培养的研 Jin ZQ,Li PL.High cell density culture of Lactobacillus del- 究.中国调味品，2004(5)：17) beueckii subsp.bulgaricus S in fed-batch fermentation.China [13]Xiong T,Xu L R,Fan L,et al.Investigation on dense cultiva- Daiy1md,2007,35(1):4 tion of Lactobacillus specific for vegetable fermentation.Food Sci, (新志强，李平兰.补料分批法高密度培养德氏乳杆菌保加利 2007,28(12):345 亚亚种S1.中国乳品工业，2007,35(1)：4) (熊涛，徐立荣，范镭，等.蔬菜发酵专用乳酸菌的菌体高密 8]Dong XZ,Cai M Y.Manual of Systematic Methods of Determina- 度培养食品科学，2007,28(12)：345) tire Bacterial.Beijing:Science Press,2001 [14]Tango M S A,Ghaly A E.Effect of temperature on lactic acid (东秀珠，蔡妙英.常见细菌系统鉴定手册.北京：科学出版 production from cheese whey using Lactobacillus helveticus under 社，2001) batch conditions.Biomass Bioenergy,1999,16(1):61 Wang Y X,Chen QS,Yan Y L.Screen and application of a few 15] Idris A,Suzana W.Effect of sodium alginate concentration,bead culture media in isolation and identification Lactobacillus.Food diameter,initial pH and temperature on lactic acid production Sci,2007,28(9):374 from pineapple waste using immobilized Lactobacillus delbrueckii. (王友湘，陈庆森，阁亚丽.用于乳酸菌分离鉴定的几种培养 Process Biochem,2006,41(5)1117 基的筛选及应用.食品科学，2007,28(9)：374) ū6] Ohkouchi Y,Inoue Y.Direet production of L(+)actic acid [10]Sun S Q,Shi J G,Li X M,et al.The determination of L-actic from starch and food wastes using Lactobacillus manihotirorans acid content of D.LAactic acid with enzyme electrode.Shandong LMG18011.Bioresour Technol,2006,97(13)1554 Sa,2008,21(2):15 07] Bai D M,Yan Z H,Wei Q,et al.Ammonium lactate production (孙士青，史建国，李雪梅，等。酶电极法测定药用乳酸中L- by Lactobacillus lactis BME5-8M in pH-controlled fed-batch fer- 乳酸含量研究.山东科学，2008,21(2)：15) mentations.Biochem Eng J,2004,19(1):47 [11]Gu C W,LiT Y,Chang Q C,et al.Dog source Lactobacillus of [18]Mu W M,Liu F L,Jia J H,et al.3-Phenyllactic acid production high density cultural conditional optimize.Chin J Microecol, by substrate feeding and pH-control in fed-batch fermentation of 2007,19(3):251 Lactobacillus sp.SK007.Bioresour Technol,2009,100 (21): (谷长维，李太元，常巧呈，等.犬源乳酸菌高密度培养条件 5226 的优化.中国微生态学杂志，2007,19(3)：251) 19]Ding SF,Tan T W.LHactic acid production by Lactobacillus ca- [12]Xiong X H,Yu X J,Xiong Q,et al.High cell density culture of sei fermentation using different fed-batch feeding strategies. lactic acid bacteria starter.Chin Condiment,2004(5):17 Process Biochem,2006,41(6):1451第 5 期 吕 乐等: 乳酸菌 USTB--08 的高效培养和生产乳酸 Process Biochem，2006，41( 1) : 120 ［7］ Jin Z Q，Li P L． High cell density culture of Lactobacillus del￾beueckii subsp． bulgaricus S-1 in fed-batch fermentation． China Dairy Ind，2007，35( 1) : 4 ( 靳志强，李平兰． 补料分批法高密度培养德氏乳杆菌保加利 亚亚种 S-1． 中国乳品工业，2007，35( 1) : 4) ［8］ Dong X Z，Cai M Y． Manual of Systematic Methods of Determina￾tive Bacterial． Beijing: Science Press，2001 ( 东秀珠，蔡妙英． 常见细菌系统鉴定手册． 北京: 科学出版 社，2001) ［9］ Wang Y X，Chen Q S，Yan Y L． Screen and application of a few culture media in isolation and identification Lactobacillus． Food Sci，2007，28( 9) : 374 ( 王友湘，陈庆森，阎亚丽． 用于乳酸菌分离鉴定的几种培养 基的筛选及应用． 食品科学，2007，28( 9) : 374) ［10］ Sun S Q，Shi J G，Li X M，et al． The determination of L-lactic acid content of D． L-lactic acid with enzyme electrode． Shandong Sci，2008，21( 2) : 15 ( 孙士青，史建国，李雪梅，等． 酶电极法测定药用乳酸中 L- 乳酸含量研究． 山东科学，2008，21( 2) : 15) ［11］ Gu C W，Li T Y，Chang Q C，et al． Dog source Lactobacillus of high density cultural conditional optimize． Chin J Microecol， 2007，19( 3) : 251 ( 谷长维，李太元，常巧呈，等． 犬源乳酸菌高密度培养条件 的优化． 中国微生态学杂志，2007，19( 3) : 251) ［12］ Xiong X H，Yu X J，Xiong Q，et al． High cell density culture of lactic acid bacteria starter． Chin Condiment，2004( 5) : 17 ( 熊晓辉，于修鉴，熊强，等． 乳酸菌发酵剂高密度培养的研 究． 中国调味品，2004( 5) : 17) ［13］ Xiong T，Xu L R，Fan L，et al． Investigation on dense cultiva￾tion of Lactobacillus specific for vegetable fermentation． Food Sci， 2007，28( 12) : 345 ( 熊涛，徐立荣，范镭，等． 蔬菜发酵专用乳酸菌的菌体高密 度培养． 食品科学，2007，28( 12) : 345) ［14］ Tango M S A，Ghaly A E． Effect of temperature on lactic acid production from cheese whey using Lactobacillus helveticus under batch conditions． Biomass Bioenergy，1999，16( 1) : 61 ［15］ Idris A，Suzana W． Effect of sodium alginate concentration，bead diameter，initial pH and temperature on lactic acid production from pineapple waste using immobilized Lactobacillus delbrueckii． Process Biochem，2006，41( 5) : 1117 ［16］ Ohkouchi Y，Inoue Y． Direct production of L ( + ) -lactic acid from starch and food wastes using Lactobacillus manihotivorans LMG18011． Bioresour Technol，2006，97( 13) : 1554 ［17］ Bai D M，Yan Z H，Wei Q，et al． Ammonium lactate production by Lactobacillus lactis BME5-18M in pH-controlled fed-batch fer￾mentations． Biochem Eng J，2004，19( 1) : 47 ［18］ Mu W M，Liu F L，Jia J H，et al． 3-Phenyllactic acid production by substrate feeding and pH-control in fed-batch fermentation of Lactobacillus sp． SK007． Bioresour Technol，2009，100 ( 21 ) : 5226 ［19］ Ding S F，Tan T W． L-lactic acid production by Lactobacillus ca￾sei fermentation using different fed-batch feeding strategies． Process Biochem，2006，41( 6) : 1451 ·607·