uestion 5 a)a plasmid is a.(Circle your answer(s). )2pts bacterium circular piece of DNA purpose enzyme petri pl vesicle b)Match each vector feature with its function. Not all answers need be used. 8 pts estriction site A)Required for expression of insert B)Allows for insertion of dna into vector E_Origin of replication C) Encodes an enzyme to cut DNA D)Enables selectability for strain that has taken up the vector Promote E) Required for duplication of vect F)Required for SRP to bind Drug resistance G Site for ribosome to bind c) To obtain the gene that rescues a tryptophan biosynthesis E coli mutant strain named, Ny-trp-Zup, you construct a genomic library from wild-type E coli by cutting the genome with BamHI and inserting the fragments into pGoSox ! a plasmid which has been very successful in the lab. pGoSox! contains the genes for tetracycline and kanamycin resistances and has a unique Bam HI restriction site that maps to the kanamycin resistance gene. you transform the library into Ny-trp- Zup and plate the transformants onto rich agar medium. you replica plate the colonies onto different media shown below Below are the plates shown in the same orientation after colonies form Rich Medium Minimal medium Rich+Tetracycline Rich +Kan (lacks tryptophan) i)Which colony(ies)contain the original pGosox!? 3 pts 1@ None 2 ii Which colony(ies)carry pGoSoX! containing an insert? 4 pts 2@ ii Which colony (ies) would you choose to further study the gene encoding the tryptophan biosynthetic enzyme that is deficient in Ny-trp-Zup? 3 pts 3@ None10 Question 5 a) A plasmid is a… (Circle your answer(s).) 2pts bacterium circular piece of DNA cell multipurpose enzyme petri plate vesicle b) Match each vector feature with its function. Not all answers need be used. 8 pts ____B____Restriction site A) Required for expression of insert B) Allows for insertion of DNA into vector ____E____Origin of replication C) Encodes an enzyme to cut DNA D) Enables selectability for strain that has taken up the vector ____A____Promoter E) Required for duplication of vector F) Required for SRP to bind ____D____Drug resistance G) Site for ribosome to bind c) To obtain the gene that rescues a tryptophan biosynthesis E.coli mutant strain named, NY-trp-Zup, you construct a genomic library from wild-type E.coli by cutting the genome with BamHI and inserting the fragments into pGoSOX!, a plasmid which has been very successful in the lab. pGoSox! contains the genes for tetracycline and kanamycin resistances and has a unique Bam HI restriction site that maps to the kanamycin resistance gene. You transform the library into NY-trp-Zup and plate the transformants onto rich agar medium. You replica plate the colonies onto different media shown below. Below are the plates shown in the same orientation after colonies form. Rich Medium Minimal medium Rich +Tetracycline Rich +Kanamycin (lacks tryptophan) i) Which colony (ies) contain the original pGoSOX!? 3 pts 1@ None 1 2 3 4 5 6 ii) Which colony (ies) carry pGoSOX! containing an insert? 4 pts 2@ None 1 2 3 4 5 6 iii) Which colony (ies) would you choose to further study the gene encoding the tryptophan biosynthetic enzyme that is deficient in NY-trp-Zup? 3 pts 3@ None 1 2 3 4 5 6 1 2 3 4 5 6