上泽充通大￥ Shanghai Jiao Tong University Important term of heat sterilization ©Thermal death time(TDT)热致死时间 the time at which all cells are killed at a given temperature Measurement:heating samples of a cell suspension for different times,mixing the heated suspensions with culture medium,incubating and check growth o Shortcoming:such destruction is logarithmic,and it is theoretically not possible to "completely destroy" microorganisms in a sample..热致死是对数性的，理论上无 法“完全杀死”样品中所有微生物 Shanghai Jiao Tong UniversityShanghai Jiao Tong University Thermal death time (TDT)热致死时间 • the time at which all cells are killed at a given temperature • Measurement: heating samples of a cell suspension for different times, mixing the heated suspensions with culture medium, incubating and check growth • Shortcoming: such destruction is logarithmic, and it is theoretically not possible to “completely destroy” microorganisms in a sample.热致死是对数性的,理论上无 法“完全杀死”样品中所有微生物 Important term of heat sterilization