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C. Yeang et aL/ Biochimica et Biophysica Acta 1781(2008)610-617 [9 A.H. Schweizer, Clausen, G. van Meer, H.P. Hauri, Localization of O-glycan authors thn was supported by NIH HL-64735 and HL-69817.The This we respect to the endoplasmic reticulum-Golgi intermediate compartment. 」 BioL Chem.269(1994)4035-4041. Dr. Zhiguo Liu from the Shanghai Institute of Organic [101 M. Malgat, A. Maurice. J. Baraud, Sphingomyelin and ceran Chemistry for his help on the homology modeling of the human SMS1 es and plasma membranes from rat liver and brain. [111 P. Moreau, C. Cassagne, Phospholipid trafficking and membrane biogenesis him. Biophys. Acta 1197(1994)257-290. References [12 M ]. Obradors, he subcellular sites of sphingomyelin nthesis in BHK cells, Biochim. Biophys. Acta. 1359(1997)1-12. [11 D.R. Voelker, E.P. Kennedy, Cellular and enzymic synthesis of sphingomyelin, Bio- [13 D. Allan, M. Obradors, Enzyme distributions in infected with Semliki forest virus: evidence for a major fraction of sphing [2]Z.Li, T.K. Hailemariam, H. Zhou, Y. Li, D.C. Duckworth, D.A. Peake, Y. Zhang, M.S. yelin synthase in the trans-Golgi network. Biochim. Biophys. Acta. 1450(1999) tracellular sphingomyelin accumulation and plasma membrane lipid organiza- [14] E Albi, M P Magni, Sphingomyelin synthase in rat liver nuclear membrane and [ 3] T Ding, ZLi, T. Ha Mukherjee, F.R. Maxfield, M P Wu, X C Jiang, SMS [15] E Albi, M.P. Magni, Chromatin-associated in: metabolism in relation [16 E Albi, S Pieroni, M P Magni, C Sartori, Chromatin sphingomyelin changes in cell 4 F.G. Tafesse, K Huitema, M. Hermansson, S. van der Poel J. van den Dikkenberg, A. proliferation and/or apoptosis induced by ciprofibrate. Cell Physiol. 196(2003) S2 are required for sphingomyelin homeostasis and growth in human HeLa [17 K Huitema. ]. van den Dikkenberg, J.F. Brouwers, J.C. Holthuis, Identification of a s. I BioL chem282(2007)17537-17547. 23(2004)33-44 [5 M. Villani, M, Subathra, Y.B. Im, Y. Choi, P. Signorelli, M. Del Poeta, C. Luberto. [18 D Halter, S. Neumann, S M. van Dijk, ]. Wolthoorn, A.M. de Maziere, o V Vieira, P. Sphingomyelin synthases regulate production of diacylglycerol at the golgi. attjus, I Klumperman, G. van Meer, H Sprong, Pre- and post-Golgi translocation lucosylceramide in glycosphingolipid synthesis, I Cell BioL. 179(2007) [6] D Separovic, L Semaan, AL Tarca, M.Y. Awad Maitah, K Hanada, J Bielawski, M. illani, C Luberto, Suppression of sphingomyelin synthase 1 by small interference [19 A.F. Neuwald, An unexpected structural relationship between integral mem- photodamage, Exp Cell Res 314 (2008)1860-1868. [7 D Separovic, K Hanada, M Y. Maitah, B Nagy, L Hang, M-A. Tainsky, J.M. Kraniak]. [20 Qx Zhang, CS. Pilquil, J Dewald, Lo ielawski, Sphingomyelin synthase 1 suppresses ceramide pro ructurally important domains of ate phosphatase-1: implications ost-photodamage, Biochem Biophys Res Commun. 358(2007)196-202. [8] D Jeckel, A Karrenbauer, R. Birk, RR. Schmidt, F. wieland, Sphingomyelin is [211 C D. Thanos, K E Goodwill, J.U. Bowie, Oligomeric structure of the human EphB2 thesized in the cis Golgi, FEBS Lett. 261(1990)155-157. receptor SAM domain, Science 283 (1999)833-836Acknowledgments This work was supported by NIH HL-64735 and HL-69817. The authors thank Dr. Zhiguo Liu from the Shanghai Institute of Organic Chemistry for his help on the homology modeling of the human SMS1 structure. References [1] D.R. Voelker, E.P. Kennedy, Cellular and enzymic synthesis of sphingomyelin, Bio￾chemistry 21 (1982) 2753–2759. [2] Z. Li, T.K. Hailemariam, H. Zhou, Y. Li, D.C. Duckworth, D.A. Peake, Y. Zhang, M.S. Kuo, G. Cao, X.C. 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