Life cycle Humans acquire E histolytica by Ingesting cysts(4 nuclei mature) in fecally contaminated food or water Rarely by directly inoculating trophozoites into colon or other sites (anal sex?) Fecal-Oral transmission(hand to mouth)Life cycle • Humans acquire E. histolytica by: – Ingesting cysts (4 nuclei mature) in fecally contaminated food or water – Rarely by directly inoculating trophozoites into colon or other sites – (anal sex?) • Fecal-Oral transmission (hand to mouth)