350 2001,15(6):345~350 [3] Thompson A E, et al. Yield potential of lesgerella( Gray) wats. a new desert plant resource for hydroxy fatty acids. Joumal of Envir onments.1989,16:331~336 [4 Smith jr CR, et al. Lesquerolic acid, a new hydroxy acid fiom Lesqrrella seed oil Jourmal of Organic Chemistry, 1961, 26: 2903- [5 Thompson A E, et al. Lesquerella -a new and land industrial oil seedcrop. guayule, 1988, 10: 16-18 [6] Gentry HS, Barclay A S. The search for new industrial cops I: prospectus of Lesguerellafendler. Ecommic Botany, 1962, 16: 206- 7 Skdar SR, et al. Enussica, the intergeneric somatic hybrid developed through protoplast fusion between Erica sativa lam and Brassica juncea(L )Czen Theor Appl Genet, 1990, 79: 561-567 [8 Rollins R C, et al. The genus Lesgnuerella(cruciferace) in north America. Harvard University Press, Cambridge MA, 1973 9]Gimelius K. Physiol. Plant, 1984, 61: 38 WILD INDUSTRIAL OIL PLANT SPECIES IMPRO VED BY PROTOPLAST FUSION BETWEEN L fendleri AN B napus YU Qiurcheng YAN Jiarming (Institue d Atomic Energ for Application in Agriculture, Jiangsu Academy d agricutural Sciences, Nanying, Jiangsu po. 210014) ABSTRACT: The protoplast fusion bet ween wild plant species L. fendleri of the genus Lesquerella and B was studied in order to transfer the industrial oil gene in L. fendleri into B The results sho wed that the percentage of calli derived from protoplats isola ted from cotyledon was lower than the that isolated from hypocotyls when the protoplasts were cultured in P& medium. The percentage of protoplat fusion treated with y-ray was higher than the that treated with PEG The hybrid plant regeneration from fusion of protoplasts bet ween L. fendleri and B. napus was the highest when the calli were cultured in B medium + 2mgZT/L. The height of hy brid plants was 60 70cm. The sha pe of leaves resembled more to those of L. fendleri; and the leaves were three times larger than L. fendleri s. The anther size and the stem he ight of the hy brid plants were ir termediate bet ween both parents. The flower type and colour were the sa me as B. napus. No seeds was obta ined beca use no pollens produced in the anther. Key words :callus; protoplast fusion; regeneration 301994-2008ChinaAcademicJournalElectronicPublishingHouseAllrightsreservedhtp/hwww.cnkinet[ 3 ] Thompson A E ,et al. Yield potential of lesqerella ( Gray) wats. ,a new desert plant resource for hydroxy fatty acids.Journal of Envir2 onments. 1989 ,16 :331～336 [ 4 ] Smith jr C R ,et al.Lesquerolic acid ,a new hydroxy acid from Lesqerella seed oil. Journal of Organic Chemistry ,1961 ,26 :2903～ 2905 [ 5 ] Thompson A E ,et al. Lesquerella —a new arid land industrial oil seedcrop. Elguayulero ,1988 ,10 :16～18 [ 6 ] Gentry H S ,Barclay A S. The search for new industrial crops Ⅲ:prospectus of Lesquerella fendleri. Economic Botany ,1962 ,16 :206～ 211 [ 7 ] Sikdar S R ,et al. Erussica ,the intergeneric somatic hybrid developed through protoplast fusion between Erica sativa lam. and Brassica juncea (L. ) Czern. Theor Appl Genet ,1990 ,79 :561～567 [ 8 ] Rollins R C ,et al. The genus Lesquerella (cruciferace) in north America. Harvard University Press ,Cambridge ,MA ,1973 , [ 9 ] Glimelius K. Physiol. Plant ,1984 ,61 :38 WILD INDUSTRIAL OIL PLANT SPECIES IMPROVED BY PROTOPLAST FUSION BETWEEN L . fendleri AND B. napus YU Qiu2cheng YAN Jian2ming ( Institute of Atomic Energy for Application in Agriculture , Jiangsu Academy of Agricultural Sciences , Nanjing , Jiangsu prov. 210014) ABSTRACT :The protoplast fusion between wild plant species L . fendleri of the genus Lesquerella and B. napus was studied in order to transfer the industrial oil gene in L . fendleri into B. napus. The results showed that the percentage of calli derived from protoplats isolated from cotyledon was lower than the that isolated from hypocotyls when the protoplats were cultured in P8 medium. The percentage of protoplat fusion treated withγ2ray was higher than the that treated with PEG. The hybrid plant regeneration from fusion of protoplasts between L . fendleri and B. napus was the highest when the calli were cultured in B5 medium + 2mgZTΠL. The height of hybrid plants was 60 ～70cm. The shape of leaves resembled more to those of L . fendleri ;and the leaves were three times larger than L . fendleri’s. The anther size and the stem height of the hybrid plants were in2 termediate between both parents. The flower type and colour were the same as B. napus. No seeds was obtained because no pollens produced in the anther. Key words :callus; protoplast fusion; regeneration 053 Acta Agriculturae Nucleatae Sinica 2001 ,15 (6) :345～350 © 1994-2008 China Academic Journal Electronic Publishing House. All rights reserved. http://www.cnki.net