Investigation of Protein Ligand Interactions by NMR EMBO Course 2007 Fast ko>>(Vee-Vpound） A+B KnAB K得 <<(Vfree -Vbound) Investigation of Protein Ligand Interactions by NMR Helen Mott University of Cambridge EMBO Course 2007 Slow Fast !"free !"bound A + B AB kon koff koff ~ (!free - !bound ) koff << (!free - !bound) koff >> (!free - !bound ) KD = [A][B] = koff [AB] kon