(3)Master effects of norepinephrine and acetylcholine on blood pressure and vascular smooth musclecontraction,and knowing tonometry and analysis of vascular strip in vitro. Experiment 12:Observation of insulin-induced hypoglycemia in rabbit and mouse 1.Teaching content Observation of insulin-induced hypoglycemic convulsion and remedy in rabbit and mouse. 2.Teaching objective (1)Understand general manifest of mammalian hypoglycemia; (2)Be familiar with main clinical application of insulin; (3)Master action mechanisms of insulin-induced hypoglycemia. Experiment 13:Effects of some drugs on isolated frog heart perfusion 1.Teaching content Preparation of frog heart perfusion in vitro;Setup of experimental system;Connection of perfusion devices;Record of normal heart-beat curve;Effects of NaCl,Cacl2, KC1,epinephrine,acetylcholine and pH value on curve. 2.Teaching objective (1)Understand preparing method of frog heart in vitro; (2)Be familiar with technique of frog heart perfusion in vitro: (3)Master responses of isolated frog heart rate and heartbeat to above-mentioned chemicals and know their action mechanism. 4.Examination manner and requirement 1.Examination manner (1)Experiment examination manner includes three parts:experiment report,experiment operation and experiment theory.（3）Master effects of norepinephrine and acetylcholine on blood pressure and vascular smooth musclecontraction, and knowing tonometry and analysis of vascular strip in vitro. Experiment 12：Observation of insulin-induced hypoglycemia in rabbit and mouse 1. Teaching content Observation of insulin-induced hypoglycemic convulsion and remedy in rabbit and mouse. 2. Teaching objective （1）Understand general manifest of mammalian hypoglycemia; （2）Be familiar with main clinical application of insulin; （3）Master action mechanisms of insulin-induced hypoglycemia. Experiment 13：Effects of some drugs on isolated frog heart perfusion 1. Teaching content Preparation of frog heart perfusion in vitro; Setup of experimental system; Connection of perfusion devices; Record of normal heart-beat curve; Effects of NaCl, CaCl2, KCl, epinephrine, acetylcholine and pH value on curve. 2. Teaching objective （1）Understand preparing method of frog heart in vitro; （2）Be familiar with technique of frog heart perfusion in vitro; （3）Master responses of isolated frog heart rate and heartbeat to above-mentioned chemicals and know their action mechanism. 4. Examination manner and requirement 1. Examination manner （1）Experiment examination manner includes three parts: experiment report, experiment operation and experiment theory