Culture of Haemophilus influenzae Requirement of X+v factor in cultivation media X= hematin, V= nicotinamide adenine dinucleotide(nad) PART 1 Growth on chocolate agar but not on blood agar Biological (when cultivated in pure culture characteristics Satelite phenomenon with Staphylococcus aureus (on blood agar) Medical MicrobiologyRequirement of X+V factor in cultivation media X = hematin, V = nicotinamide adenine dinucleotide(NAD) Growth on chocolate agar but not on blood agar (when cultivated in pure culture) Satelite phenomenon with Staphylococcus aureus (on blood agar) Culture of Haemophilus influenzae PART 1 Biological characteristics Medical Microbiology