D0L:10.13374.issn1001-053x.2013.10.005 第35卷第10期 北京科技大学学报 Vol.35 No.10 2013年10月 Journal of University of Science and Technology Beijing 0ct.2013 胶质芽孢杆菌HJO7的UV与NTG诱变育种及 其对铝土矿浸矿效果 孙德四1,2)☒，王化军2)，张强2) 1)九江学院化学与环境工程学院，九江3320052)北京科技大学土木与环境工程学院，北京100083 冈通信作者，E-mail:sundesi1215@126.com 摘要以从河南铝土矿样筛选出的一株胶质芽孢杆菌HJ07为出发菌株，对其进行紫外(UV)与亚硝基胍(NTG)诱 变育种及铝土矿浸矿脱硅研究.分别通过紫外线照射120s与采用质量浓度为600gL~1的亚硝基胍处理，出发菌株 HJ07的致死率分别达到89%与90%，正突变率分别达到16.5%与18.7%.从突变菌株中筛选所得的两株菌种UV-2与 NTG-5的生长代谢活性与脱硅能力明显比出发菌株高.在铝土矿浸出体系中，UV-2与NTG5达到生长稳定期的时间 比HJ07分别缩短了48h与24h,且生长稳定期具有更大的细菌浓度.浸矿12d后，UV-2与NTG-5菌株浸出液中 SiO2的质量浓度分别比H07提高了约25.6%与12.5%，且达到浸出终点的时间分别缩短了3d和2d.UV-2与NTG-5 菌株较出发菌株HJ07具有更强的产酸与产胞外聚合物的能力.被UV-2菌株作用后的铝土矿表面的溶蚀程度更加显 著，矿物表面形成了明显的菌胶团. 关键词铝土矿处理：生物浸矿：胶质芽孢杆菌：诱变 分类号TD925 Breeding of Bacillus mucilaginosus HJO7 mutated by UV/NTG and its effects on microbial leaching of bauxite SUN De-si12),WANG Hua-jun2),ZHANG Qiang?) 1)School of Chemistry and Environmental Engineering,Jiujiang University,Jiujiang 332005,China 2)School of Civil and Environmental Engineering,University of Science and Technology Beijing,Beijing 100083,China Corresponding author,E-mail:sundesi1215@126.com ABSTRACT An original strain Bacillus mucilaginosus HJ07 was screened from bauxite sampled from Henan Province of China.It was induced mutagenesis by ultraviolet (UV)radiating and nitrosoguanidine (NTG)culturing,and then was used for bioleaching silicon from bauxite.After treated by 120s UV radiating and 600 mg-L-NTG culturing, its kill rates are 89%and 90%,and its positive mutation rates are 16.5%and 18.7%,respectively.Two mutant strains UV-2 and NTG-5 were screened from a large number of mutant strains,and they have better bioactivity and de-silicon efficiency than HJ07 strain.In comparison with HJ07 strain,the time that UV-2 and NTG-5 strains reach stable growth in bauxite bioleaching systems shortens by 48 h and 24 h,respectively,and they have higher bacterial concentrations. After 12 d bioleaching,SiO2 mass concentrations in leachates of UV-2 and NTG-5 stains improve by about 25.6%and 12.5%,and the time that UV-2 and NTG-5 strains reach the leaching terminal point shortens by 3d and 2 d,respectively. UV-2 and NTG-5 strains can produce much more organic acids and extracellular polymeric substances than HJ07 strain. Moreover,mineral particle surfaces leached by UV-2 strain are eroded much more obviously and covered with a thicker layer of zooglea. 收稿日期：2012-08-24 基金项目：因家自然科学基金资助项目(51064011：5126401431360064)第 35 卷 第 10 期 北 京 科 技 大 学 学 报 Vol. 35 No. 10 2013 年 10 月 Journal of University of Science and Technology Beijing Oct. 2013 胶质芽孢杆菌 HJ07 的 UV 与 NTG 诱变育种及 其对铝土矿浸矿效果 孙德四1,2) ，王化军2)，张 强2) 1) 九江学院化学与环境工程学院，九江 332005 2) 北京科技大学土木与环境工程学院，北京 100083 通信作者，E-mail: sundesi1215@126.com 摘 要 以从河南铝土矿样筛选出的一株胶质芽孢杆菌 HJ07 为出发菌株，对其进行紫外 (UV) 与亚硝基胍 (NTG) 诱 变育种及铝土矿浸矿脱硅研究. 分别通过紫外线照射 120 s 与采用质量浓度为 600 mg·L −1 的亚硝基胍处理，出发菌株 HJ07 的致死率分别达到 89%与 90%，正突变率分别达到 16.5%与 18.7%. 从突变菌株中筛选所得的两株菌种 UV-2 与 NTG-5 的生长代谢活性与脱硅能力明显比出发菌株高. 在铝土矿浸出体系中，UV-2 与 NTG-5 达到生长稳定期的时间 比 HJ07 分别缩短了 48 h 与 24 h，且生长稳定期具有更大的细菌浓度. 浸矿 12 d 后，UV-2 与 NTG-5 菌株浸出液中 SiO2 的质量浓度分别比 HJ07 提高了约 25.6%与 12.5%，且达到浸出终点的时间分别缩短了 3 d 和 2 d. UV-2 与 NTG-5 菌株较出发菌株 HJ07 具有更强的产酸与产胞外聚合物的能力. 被 UV-2 菌株作用后的铝土矿表面的溶蚀程度更加显 著，矿物表面形成了明显的菌胶团. 关键词 铝土矿处理；生物浸矿；胶质芽孢杆菌；诱变 分类号 TD925 Breeding of Bacillus mucilaginosus HJ07 mutated by UV/NTG and its effects on microbial leaching of bauxite SUN De-si 1,2) , WANG Hua-jun2), ZHANG Qiang2) 1) School of Chemistry and Environmental Engineering, Jiujiang University, Jiujiang 332005, China 2) School of Civil and Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, China Corresponding author, E-mail: sundesi1215@126.com ABSTRACT An original strain Bacillus mucilaginosus HJ07 was screened from bauxite sampled from Henan Province of China. It was induced mutagenesis by ultraviolet (UV) radiating and nitrosoguanidine (NTG）culturing, and then was used for bioleaching silicon from bauxite. After treated by 120 s UV radiating and 600 mg·L −1 NTG culturing, its kill rates are 89% and 90%, and its positive mutation rates are 16.5% and 18.7%, respectively. Two mutant strains UV-2 and NTG-5 were screened from a large number of mutant strains, and they have better bioactivity and de-silicon efficiency than HJ07 strain. In comparison with HJ07 strain, the time that UV-2 and NTG-5 strains reach stable growth in bauxite bioleaching systems shortens by 48 h and 24 h, respectively, and they have higher bacterial concentrations. After 12 d bioleaching, SiO2 mass concentrations in leachates of UV-2 and NTG-5 stains improve by about 25.6% and 12.5%, and the time that UV-2 and NTG-5 strains reach the leaching terminal point shortens by 3 d and 2 d, respectively. UV-2 and NTG-5 strains can produce much more organic acids and extracellular polymeric substances than HJ07 strain. Moreover, mineral particle surfaces leached by UV-2 strain are eroded much more obviously and covered with a thicker layer of zooglea. 收稿日期：2012-08-24 基金项目：国家自然科学基金资助项目 (51064011; 51264014; 31360064) DOI:10.13374/j.issn1001-053x.2013.10.005