维进,等剧源干细胞在组织工程和再生医学的应用 Www.crter. org 264 Hospital of Chinese PLA. BACKGROUND: Muscle satellite cells are a main cell source for the repair of muscle tissue injury, and have been aiyuan 030001, shan id extensive attention in the study of muscle tissue regeneration rovince. China: Plastic and OBJECTIVE: To summarize the biological properties of muscle-derived stem cells and its application in the field Changzheng Hospital, Second of tissue engineering and regenerative medicine, and to explore the clinical application value and perspective of Miitary Medical University of METHODS: We retrieved Vip database and PubMed database for articles concerning the application of hina, Neurosaence Research muscle-derived stem cells published from January 2000 to April 2011. In titles and abstracts, the key words were enter, Changzheng Hospital, econd Military Medical muscle-derived stem cells, muscle satellite cells, tissue engineering, regenerative medicine, adult stem cells MDSCs, MSCs, ASCs" Articles addressing the application of muscle-derived stem cells were included, and those 200003 China in the same field published recently or in authorized joumals were included. 189 literatures were primarily collected, and a total of 25 literatures were included according to the inclusion criteria RESULTS AND CONCLUSION: Animal experiments and success clinical experiments suggested that in Corresponding author. Jiang Hua, Aesthetic Plastic Surger combination of genetic engineering and tissue engineering, tissue engineered tissues and cytological therapy based on muscle-derived stem cells can be used in various skeletal muscle diseases, myocardial diseases urinary system and nervous system by safe measures. This provides a good healing perspective for clinical 30001 Shanxi Province physicians and patients, but the study of muscle-derived stem cells still faced many problems. China: Plastic and Key Words: stem cells; stem cell academic discussion: muscle-derived stem cells; muscle satellite cells; tissue wangzheng Hospital, Second engineering; regenerative medicine: adult stem cells; isolation and culture; bone tissue engineering; vascular regeneration Ding WJ, Su ZD, Jiang H. Application of muscle-derived stem cells in tissue engineering and regenerative medicine. Zhongguo Zuzhi Gongcheng Yanjiu 2013: 17(23): 4328-4333 Accepted: 2013-0 0引言 近来从人体肌组织分离得到肌源干细胞为该领域的硏究带来新方向,拉近了肌源干 细胞和临床应用之间的距离。以肌源干细胞为基础的组织工程学和再生医学研究,为临 床治疗各种骨骼肌疾病、心肌疾病、泌尿系统和神经系统的疾病提供了令人振奋的前景, 现就肌源干细胞国内外研究现状进行综述。 资料和方法 1.1资料来源由笫一作者在2011年4月进行检索。检索数据库: PubMed数据库,网址 htt/www.ncbi.nim.gov/pubmed;维普数据库,网址htp/www.vmis.net.cn/yixue ndex.asp。检索时间范围为1999年1月至2011年4月。英文检索词为“ MDSCS,MSCs AsCs, regenerative medicine; tissue engineering”;中文检索词为“肌源干细胞,肌卫 星细胞,组织工程,再生医学,成体干细胞 1.2入选标准纳入标准:①肌源干细胞的基础和临床研究。②肌源干细胞的生物学特 点。③肌源干细胞在组织工程和再生医学研究中的应用。排除标准:①与此文目的无关 ②较陈旧的文献。③重复同类研究。 1.3质量评估文献筛选和质量评价由2位研究者独立进行并交叉核对,独立使用统一资 料提取数据,遇有分歧讨论解决 2结果 2.1纳入文献基本情况初检得到189篇文献,中文80篇,荚文109篇。阅读标题和摘要 /SSN 2095-4344 CN 21-1581/R CODEN: ZLKHAH 4329 C1994-2013ChinaAcademicJournalElectronicPublishingHouse.Allrightsreservedhttp://www.cnki.net丁维进，等. 肌源干细胞在组织工程和再生医学的应用 ISSN 2095-4344 CN 21-1581/R CODEN: ZLKHAH 4329 www.CRTER.org Ding Wei-jin☆, M.D., Aesthetic Plastic Surgery Department, the 264 Hospital of Chinese PLA, Taiyuan 030001, Shanxi Province, China; Plastic and Reconstructive Department, Changzheng Hospital, Second Military Medical University of PLA, Shanghai 200003, China; Neuroscience Research Center, Changzheng Hospital, Second Military Medical University of PLA, Shanghai 200003, China dingweijin@yahoo.cn Corresponding author: Jiang Hua, Aesthetic Plastic Surgery Department, the 264 Hospital of Chinese PLA, Taiyuan 030001, Shanxi Province, China; Plastic and Reconstructive Department, Changzheng Hospital, Second Military Medical University of PLA, Shanghai 200003, China Received: 2013-01-12 Accepted: 2013-02-29 Abstract BACKGROUND: Muscle satellite cells are a main cell source for the repair of muscle tissue injury, and have been paid extensive attention in the study of muscle tissue regeneration. OBJECTIVE: To summarize the biological properties of muscle-derived stem cells and its application in the field of tissue engineering and regenerative medicine, and to explore the clinical application value and perspective of muscle-derived stem cells. METHODS: We retrieved Vip database and PubMed database for articles concerning the application of muscle-derived stem cells published from January 2000 to April 2011. In titles and abstracts, the key words were “muscle-derived stem cells, muscle satellite cells, tissue engineering, regenerative medicine, adult stem cells, MDSCs, MSCs, ASCs”. Articles addressing the application of muscle-derived stem cells were included, and those in the same field published recently or in authorized journals were included. 189 literatures were primarily collected, and a total of 25 literatures were included according to the inclusion criteria. RESULTS AND CONCLUSION: Animal experiments and success clinical experiments suggested that in combination of genetic engineering and tissue engineering, tissue engineered tissues and cytological therapy based on muscle-derived stem cells can be used in various skeletal muscle diseases, myocardial diseases, urinary system and nervous system by safe measures. This provides a good healing perspective for clinical physicians and patients, but the study of muscle-derived stem cells still faced many problems. Key Words: stem cells; stem cell academic discussion; muscle-derived stem cells; muscle satellite cells; tissue engineering; regenerative medicine; adult stem cells; isolation and culture; bone tissue engineering; vascular regeneration Ding WJ, Su ZD, Jiang H. Application of muscle-derived stem cells in tissue engineering and regenerative medicine.Zhongguo Zuzhi Gongcheng Yanjiu. 2013;17(23):4328-4333. 0 引言 近来从人体肌组织分离得到肌源干细胞为该领域的研究带来新方向，拉近了肌源干 细胞和临床应用之间的距离。以肌源干细胞为基础的组织工程学和再生医学研究，为临 床治疗各种骨骼肌疾病、心肌疾病、泌尿系统和神经系统的疾病提供了令人振奋的前景， 现就肌源干细胞国内外研究现状进行综述。 1 资料和方法 1.1 资料来源 由第一作者在2011年4月进行检索。检索数据库：PubMed数据库，网址 http://www.ncbi.nlm.gov/PubMed ；维普数据库，网址 http://www.vmis.net.cn/yixue/ index.asp。检索时间范围为1999年1月至2011年4月。英文检索词为“MDSCs，MSCs， ASCs, regenerative medicine；tissue engineering”；中文检索词为“肌源干细胞，肌卫 星细胞，组织工程，再生医学，成体干细胞”。 1.2 入选标准 纳入标准：①肌源干细胞的基础和临床研究。②肌源干细胞的生物学特 点。③肌源干细胞在组织工程和再生医学研究中的应用。排除标准：①与此文目的无关。 ②较陈旧的文献。③重复同类研究。 1.3 质量评估 文献筛选和质量评价由2位研究者独立进行并交叉核对，独立使用统一资 料提取数据，遇有分歧讨论解决。 2 结果 2.1 纳入文献基本情况 初检得到189篇文献，中文80篇，英文109篇。阅读标题和摘要