June 2008 Journal of northeast Agricultural University ol.15No.226-29 Effects of Immunopotentiator of the Traditional Chinese Medicine on T Lymphocytes in Chicken Blood SHI Qiumei, LI Chunling GAo Guisheng, SHEN Ping, and TANG Shengling 2 Veterinary Research Institute, Guangdong Provincial Academy of Agricultural Sciences, Guangzhou 510640,China Abstract: In order to investigate the mechanism of action of immunoenhancer, the effects of the traditional Chinese medicine oter on the quantity and the transformation rates of T lymphocytes in the chicken blood were determined. Total 120 chickens were randomly assigned into three groups. The 1% and the 0.5% of the Chinese medicine immunopromoter were added to the chicken drinking water, respectively. The quantity of T lymphocytes in each group was measured by a-Naphthyl acetate esterase(ANAE)staining. The results showed that the percentages of T lymphocytes of the treatment groups were significantly higher than that of the control group(P<0.05), and the percentage of the 1% group significantly higher than that of the 0.5% group (P<0.05). In conclusion, the transformation rates of T lymphocytes showed that the Chinese medicine immunopromoter had the significant enhancing effect on the transformation rates of T lymphocytes of the treated chickens. The traditional Chinese medicine amunopromoter had the distinct function to promote the quantity and the transformation rate of T lymphocytes Key words: traditional Chinese medicine immunopromoter, T lymphocyte, a-Naphthyl acetate esterase staining CLC number: S831; S852 16 Document code: A Article ID: 1006-8104(2008)-02-0026-04 phages so that the body can obtain the high-level Introduction immune response after vaccination and strengthen the resistance to diseases. The immune function of the There are still outbreaks of infectious diseases in animal directly affects its resistance to diseases and vaccinated chickens and the gradually increasing the immune effect after vaccination. The level of the dosage fails to achieve the ideal therapeutic effects. immune function of the animal is closely related to The reason may be the decreasing immune function the quantity of T and B lymphocytes. It is generally of the body due to the long-term mindless use of accepted that the function of T lymphocytes can pro- medicine and unreasonable immune vaccination, duce lymphokines to increase the immunity and specially when the development of lymphocytes assist humoral immunity. At present, great progres and the activity of immunocytes such as peripheral has been made in the research of chemical medicine lood lymphocyte and macrophage are in a low or immunopromoters that play a certain active role highly suppressive state. To effectively solve the pro- in the healthy development of animal husbandry blem, it is necessary to activate the immune system However, these promoters have side or even toxic and increase the transformation rate of the peripheral effects and contain pollution residues. Therefore,re- blood lymphocytes, devouring capability of macro- searchers have been paying more attention to the Received 23 May 2007 Supported by Hebei Science and Technology Office Programme(07220401D) Guangdong Science and Technology Office Programme(2006B203010 1020) SHI Qiumei(1965-), female, Ph. D candidate, professor, engaged in the research of animal infectious disease E-mail: xuebaoenglisheneau. edu.cn C1994-2008ChinaAcademicJOurnalElectronicPublishingHouse.Allrightsreservedhttp:/www.cnki.netEffects of Immunopotentiator of the Traditional Chinese Medicine on T Lymphocytes in Chicken Blood SHI Qiumei1 , LI Chunling2 , GAO Guisheng1 , SHEN Ping1 , and TANG Shengling1 1 Department of Animal Sciences, Hebei Normal University of Science and Technology, Changli 066600, Hebei, China 2 Veterinary Research Institute, Guangdong Provincial Academy of Agricultural Sciences, Guangzhou 510640, China Abstract：In order to investigate the mechanism of action of immunoenhancer, the effects of the traditional Chinese medicine immunopromoter on the quantity and the transformation rates of T lymphocytes in the chicken blood were determined. Total 120 chickens were randomly assigned into three groups. The 1% and the 0.5% of the Chinese medicine immunopromoter were added to the chicken drinking water, respectively. The quantity of T lymphocytes in each group was measured by a-Naphthyl acetate esterase (ANAE) staining. The results showed that the percentages of T lymphocytes of the treatment groups were significantly higher than that of the control group (P<0.05), and the percentage of the 1% group significantly higher than that of the 0.5% group (P<0.05). In conclusion, the transformation rates of T lymphocytes showed that the Chinese medicine immunopromoter had the significant enhancing effect on the transformation rates of T lymphocytes of the treated chickens. The traditional Chinese medicine immunopromoter had the distinct function to promote the quantity and the transformation rate of T lymphocytes. Key words：traditional Chinese medicine immunopromoter, T lymphocyte, a-Naphthyl acetate esterase staining CLC number: S831; S852.16 Document code: A Article ID: 1006-8104(2008)-02-0026 -04 Introduction There are still outbreaks of infectious diseases in vaccinated chickens and the gradually increasing dosage fails to achieve the ideal therapeutic effects. The reason may be the decreasing immune function of the body due to the long-term mindless use of medicine and unreasonable immune vaccination, especially when the development of lymphocytes and the activity of immunocytes such as peripheral blood lymphocyte and macrophage are in a low or highly suppressive state[1]. To effectively solve the pro￾blem, it is necessary to activate the immune system and increase the transformation rate of the peripheral blood lymphocytes, devouring capability of macro￾phages so that the body can obtain the high-level immune response after vaccination and strengthen the resistance to diseases. The immune function of the animal directly affects its resistance to diseases and the immune effect after vaccination. The level of the immune function of the animal is closely related to the quantity of T and B lymphocytes. It is generally accepted that the function of T lymphocytes can pro￾duce lymphokines to increase the immunity and assist humoral immunity. At present, great progress has been made in the research of chemical medicine immunopromoters that play a certain active role in the healthy development of animal husbandry. However, these promoters have side or even toxic effects and contain pollution residues. Therefore, re￾searchers have been paying more attention to the Received 23 May 2007 Supported by Hebei Science and Technology Office Programme (07220401D); Guangdong Science and Technology Office Programme (2006B203010 1020) SHI Qiumei (1965-), female, Ph. D candidate, professor, engaged in the research of animal infectious disease. June 2008 Journal of Northeast Agricultural University Vol. 15 No.2 26-29 E-mail: xuebaoenglish@neau.edu.cn