Applications of NGS Category Examples of applications Complete genome resequencing Comprehensive polymorphism and mutation discovery in individual human genomes Reduced representation sequencing Large-scale polymorphism discovery Targeted genomic resequencing Targeted polymorphism and mutation discovery Paired end sequencing Discovery of inherited and acquired structural variation Metagenomic sequencing Discovery ofinfectious and commensal flora RNA-Seq Deep-sequencing shotgun libraries derived from mRNA/small RNAs.microRNA profiling/splice junctions/transcript boundaries/structural rearrangements/copy number variation DNA methylation Determining patterns of cytosine methylation in genomic DNA,large-scale analysis of DNA methylation,by deep sequencing of bisulfite-treated DNA Chromatin immunoprecipitation- Genome-wide mapping of protein-DNA interactions,by deep sequencing(ChIP-Seq) sequencing of DNA fragments pulled down by ChIP. Nuclease fragmentation and Nucleosome positioning sequencing Molecular barcoding Multiplex sequencing of samples from multiple individualsApplications of NGS Category Examples of applications Complete genome resequencing Comprehensive polymorphism and mutation discovery in individual human genomes Reduced representation sequencing Large-scale polymorphism discovery Targeted genomic resequencing Targeted polymorphism and mutation discovery Paired end sequencing Discovery of inherited and acquired structural variation Metagenomic sequencing Discovery of infectious and commensal flora RNA-Seq Deep-sequencing shotgun libraries derived from mRNA/small RNAs. microRNA profiling/splice junctions/transcript boundaries/structural rearrangements/copy number variation DNA methylation Determining patterns of cytosine methylation in genomic DNA , large-scale analysis of DNA methylation, by deep sequencing of bisulfite-treated DNA Chromatin immunoprecipitation– sequencing (ChIP-Seq) Genome-wide mapping of protein-DNA interactions, by deep sequencing of DNA fragments pulled down by ChIP. Nuclease fragmentation and sequencing Nucleosome positioning Molecular barcoding Multiplex sequencing of samples from multiple individuals