第期 赵婧等:细胞电化学分析的研究进展 47 Li T, Fan Q, Liu T, Zhu X L, Zhao J, Li GX. Biosens. Bioelectron, 2010, 25(12): 2686-2689 48 Yang Q L, Nie Y J, Zhu X L, Liu xJ, Li G x. Electrochimica Acta, 2009, 55(1): 276-280 49 Chen L, Huang J Y, Meng F B, Zhou N D. Anal. Sci., 2010, 26(5): 535-538 50 Shao Z Y. Liu YX, Xiao H, Li G X. Electrochem, Comm un., 2008, 10(10): 1502-1504 51 Yang wQ, Zhu X, Liu Q D, Lin ZY, Qiu B, Chen G N. Chem. Com mun, 2011, 47(11): 3129-3131 52 Li Y, Liu B W, Li x, Wei Q L, Biosens, Bioelectron, 2010, 25 (11):2543-2547 53 Jia X E, Tan L, Zhou Y P, Jiang X F, Xie Q J, Tang H, Yao S Z. Electrochem. Commun., 2009, 11(1) 141~144 54 Zhong X, Bai H J, XuJ J, Chen H Y, Zhu Y H. Adv. Funct. Mater, 2010, 20(6): 992-999 55Gu M, Zhang J, Li Y, Jiang L, Zhu JJ. Talanta, 2009,80(1): 246-249 56 Feng L, Chen Y, Ren J, Qu X. Biomaterials, 2011, 32(11): 2930-2937 57 Liu L, Zhu X L, Zhang D M, Huang Y, L iGx. Electrochem. Comm, 2007,9(10): 2547-2550 58 dela Escosura-Muniz A. Sanchez-Espinel C, Diaz-Freitas B. Gonzalez-Fernandez A, Maltez-da Costa M. Merkoci A Chem,,2009,81(24):10268~10274 59 El-Said WA, YeaC H, Kim H, Oh B K, Choi j W. Biosens. Bioelectron, 2009, 24(5):1259-1265 60 Wu D M, FuG L, Fang H Z, Hu L, Li J L, Yuan x, Zhang ZY. Talanta, 2009, 78(2): 602-607 61 Wu D M, Fu G L, Wang J T, Ge L Y, Zhu J H, Yuan X, Liu J G. Electrochem. Commun., 2011, 13(6) 62 Bareket L, Rephaeli A, Berkovitch G, Nudelman A, Rishpon J. Bioelectrochemistry, 2010, 77(2): 94-99 Research Progress in Electroanalysis of Cells 2( De partment of Biochemistry and State Key Laboratory of Pharmaceutical Biotechnology, china) I(Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 20044 Nanjing University, Nanjing 210093, China) Abstract As the basic units of life organism, cells play a key role in the developmental and physiolog cal process of the organism. Meanwhile, since the physiological activities of cells are usually coupled with electron transfer and/or eletroactive species transferring, electroanalytical method has proven to be a very useful technique for the study of cells, which will be beneficial to the assay of cell activity disease diagnosis and drug screening. Moreover, with the development of surface modification, nano- technology and molecular recognition, more and more modified electrodes with biocompatible surfac have been fabricated for electroanalytical study of cells, which may open more opportunities for the study of cells. Therefore, electroanalysis of cells has received more and more research interests, and lots of achievements have been obtained by the colleagues in China and those in the other countries Herein, we summarize the research progress of cell-based electroanalytical studies mostly in the recent three years to give a general overview to this field Keywords Cell; Apoptosis; Tumor cell; Electroanalysis: Review Received 24 October 2011: accepted 22 December 20110)47 LiT,FanQ,LiuT,ZhuXL,ZhaoJ,LiGX.Biosens.Bioelectron.,2010,25(12):2686~2689 48 YangQL,NieYJ,ZhuXL,LiuXJ,LiGX.ElectrochimicaActa,2009,55(1):276~280 49 ChenL,HuangJY,MengFB,ZhouND.Anal.Sci.,2010,26(5):535~538 50 ShaoZY,LiuYX,XiaoH,LiGX.Electrochem.Commun.,2008,10(10):1502~1504 51 YangW Q,ZhuX,LiuQD,LinZY,QiuB,ChenGN.Chem.Commun.,2011,47(11):3129~3131 52 LiY,LiuB W,LiX,WeiQL.Biosens.Bioelectron.,2010,25(11):2543~2547 53 JiaXE,TanL,Zhou Y P,JiangX F,XieQJ,Tang H,YaoSZ.Electrochem.Commun.,2009,11(1): 141~144 54 ZhongX,BaiHJ,XuJJ,ChenH Y,ZhuY H.Adv.Funct.Mater.,2010,20(6):992~999 55GuM,ZhangJ,LiY,JiangL,ZhuJJ.Talanta,2009,80(1):246~249 56 FengL,ChenY,RenJ,QuX.Biomaterials,2011,32(11):2930~2937 57 LiuL,ZhuXL,ZhangD M,HuangJY,LiGX.Electrochem.Comm.,2007,9(10):2547~2550 58 delaEscosura-MuñizA,S췍nchez-EspinelC,Díaz-FreitasB,Gonz췍lez-Fern췍ndezA,Maltez-daCostaM,MerkoçiA. Anal.Chem.,2009,81(24):10268~10274 59 El-SaidW A,YeaC H,Kim H,OhBK,ChoiJW.Biosens.Bioelectron.,2009,24(5):1259~1265 60 WuD M,FuGL,FangHZ,HuL,LiJL,YuanX,ZhangZY.Talanta,2009,78(2):602~607 61 WuD M,FuG L,WangJT,GeL Y,ZhuJH,YuanX,LiuJG.Electrochem.Commun.,2011,13(6): 623~626 62 BareketL,RephaeliA,BerkovitchG,NudelmanA,RishponJ.Bioelectrochemistry,2010,77(2):94~99 ResearchProgressinElectroanalysisofCells ZHAOJing 1,ZHU Xiao-Li1,LIGen-Xi*1,2 1(LaboratoryofBiosensingTechnology,SchoolofLifeSciences,ShanghaiUniversity,Shanghai200444,China) 2(DepartmentofBiochemistryandStateKeyLaboratoryofPharmaceuticalBiotechnology, NanjingUniversity,Nanjing210093,China) Abstract Asthebasicunitsoflifeorganism,cellsplayakeyroleinthedevelopmentalandphysiolog￾icalprocessoftheorganism.Meanwhile,sincethephysiologicalactivitiesofcellsareusuallycoupled withelectrontransferand/oreletroactivespeciestransferring,electroanalyticalmethodhasprovento beaveryusefultechniqueforthestudyofcells,whichwillbebeneficialtotheassayofcellactivity, diseasediagnosisanddrugscreening.Moreover,withthedevelopmentofsurfacemodification,nano￾technologyandmolecularrecognition,moreandmoremodifiedelectrodeswithbiocompatiblesurface havebeenfabricatedforelectroanalyticalstudyofcells,which mayopen moreopportunitiesforthe studyofcells.Therefore,electroanalysisofcellshasreceivedmoreandmoreresearchinterests,and lotsofachievementshavebeenobtainedbythecolleaguesinChinaandthoseintheothercountries. Herein,wesummarizetheresearchprogressofcell-basedelectroanalyticalstudies mostlyinthe recentthreeyearstogiveageneraloverviewtothisfield. Keywords Cell;Apoptosis;Tumorcell;Electroanalysis;Review (Received24October2011;accepted22December20110) 第期 赵 婧等: 细胞电化学分析的研究进展