科学无“争论”一—实验能否被重复成为关键 nature .lmage Problem International weekly journal of science Home News Comment Research Careers Jobs Current Issue Archive Audio Vide .Experimental Protocol News Comment News 2014 September Article NATURE I NEWS 炉 Acid-bath stem-cell study Experimental protocol The protocol might just be complicated-even Wakayama has been having trouble reproducing Japanese research institute launches inquiry the results.He and a student in his laboratory did replicate the experiment independently before blockbuster papers. publication,after being well coached by Obokata.But since he moved to Yamanashi,he has had David Cyranoski no luck."It looks like an easy technique-just add acid-but it's not that easy,"he says. 17 February 2014 Wakayama says that his independent success in reproducing Obokata's results is enough to Rights Permissions convince him that the technique works.He also notes that the cells produced by Obokata are the only ones known-aside from those in newly fertilized embryos-to be able to produce,for A leading Japanese research centre has opened example,placentas,so could not have been substituted cells."I did it and found it myself,"he says. an investigation into a groundbreaking stem-cell "I know the results are absolutely true." study after concerns were raised about the work's credibility. Several scientists have contacted one or more of the papers'authors for more details on the protocol,but have not yet received a response.Hongkui Deng,a stem-cell biologist at Peking The RIKEN Center for Developmental Biology in University in Beijing,was told that "the authors will publish a detailed protocol soon".Vacanti says Kobe announced on Friday that it is looking into that he has had no problem repeating the experiment but that he will let Obokata supply the protocol "to avoid any potential for variation that could lead to confusion".科学无“争论”——实验能否被重复成为关键 •Image Problem •Experimental Protocol