BEH.462/3. 962J Molecular Principles of Biomaterials Spring 2003 Gas Exchange ID DO o4 a Other Tissues Out Models retention of chemical in blood and interstitial fluid Figure 4.(a)Microscopic CCA system with four chambers. The dimensions(w x I x d of the chambers are: lung 2 mm x 2 mm x 20 um; liver 3.5 mm x 4.6 mm x 20 um; other tissue 0.4 m x 100am:fat0.42mm×50.6mm×100m Cells are cultured as monolayers on the silicon surfaces modified by adsorption of polylysine and collagen(b) ( Park and Shuler 2003 In vivo detection Biofouling typically limits lifetime of in vivo measurements to 1-2 days o Loss of vasculature Lecture 20-Biosensors 7 of 8BEH.462/3.962J Molecular Principles of Biomaterials Spring 2003 Lecture 20 – Biosensors 7 of 8 (Park and Shuler 2003) Models retention of chemical in blood and interstitial fluid In vivo detection ƒ Biofouling typically limits lifetime of in vivo measurements to 1-2 days o Inflammation o Fibrosis o Loss of vasculature