1. Will look down or look up the scale affect the volume during pipetting Question and quiz in 2.In room temperature,is the absorption proportional to the concentration of the class solution? After class 1.Emphasize the importance to balance the sample before centrifugation summary 2.Emphasize the use proper blank to calibrate spectrophotometerQuestion and quiz in class 1. Will look down or look up the scale affect the volume during pipetting 2. In room temperature, is the absorption proportional to the concentration of the solution? After class summary 1. Emphasize the importance to balance the sample before centrifugation 2. Emphasize the use proper blank to calibrate spectrophotometer