Name: TA To determine how this mutated sequence might affect the mRNa and the protein product, you obtain tw cDNA libraries: one derived from wild type cardinal RNa and one derived from diseased cardinal rna d)you choose to use a radioactively labeled DNa probe to screen these cDNA libraries for clones containing soks. Which region of soks would make the best probe for screening the available cDNA libraries?( Circle either Region A, Region B, or Region C )4 pts Region Region B Region C 100 200 400 Distances in base pairs your probe hybridizes to cDNA clones in both cDNA libraries. you purify the plasmids from single clones and cut them with the restriction enzyme used for cloning to verify insert size. The gel is shown below Ladder WT Diseased 1000bp ← Vector 700bp 500b 300 e)Based on all evidence above, what is the most likely explanation for the difference in restriction enzyme digestion patterns of the soks cDNA clones? 3 pts i)A mutation in the soks cDNa from the diseased cardinal cDNA library gives rise to an additional restriction enzyme site i The mRNA encoded by the soks gene from the diseased cardinal is incorrectly spliced ii There is likely a problem with the gel and it should be rerun. iv) The soks gene from the diseased cardinal acquired a spontaneous insertion v The mRNa encoded by the soks gene from the diseased cardinal poly A tailName:___________________________________ TA:_______________________ 5 To determine how this mutated sequence might affect the mRNA and the protein product, you obtain two cDNA libraries: one derived from wild type cardinal RNA and one derived from diseased cardinal RNA. d) You choose to use a radioactively labeled DNA probe to screen these cDNA libraries for clones containing sokS. Which region of sokS would make the best probe for screening the available cDNA libraries? (Circle either Region A, Region B, or Region C.) 4 pts Region Region B Region C 100 100 40 200 400 50 Distances in base pairs Your probe hybridizes to cDNA clones in both cDNA libraries. You purify the plasmids from single clones and cut them with the restriction enzyme used for cloning to verify insert size. The gel is shown below. Ladder WT Diseased e) Based on all evidence above, what is the most likely explanation for the difference in restriction enzyme digestion patterns of the sokS cDNA clones? 3 pts i) A mutation in the sokS cDNA from the diseased cardinal cDNA library gives rise to an additional restriction enzyme site. ii) The mRNA encoded by the sokS gene from the diseased cardinal is incorrectly spliced. iii) There is likely a problem with the gel and it should be rerun. iv) The sokS gene from the diseased cardinal acquired a spontaneous insertion. v) The mRNA encoded by the sokS gene from the diseased cardinal has no poly A tail. 1000 bp 700 bp 500 bp 300 bp 50 bp Vector 1 2 3