11.3 Essentials of Molecular Cloning Molecular cloning:isolation and incorporation of a piece of DNA into a vector so it can Foreign DNA be replicated and manipulated Cut with restriction enzyme Three main steps of gene Add vector cut Sticky with same ends restriction enzyme cloning: Vector Add DNA ligase to 1.Isolation and fragmentation form recombinant molecules of source DNA Cloned- DNA 2. Insertion of DNA fragment Introduction of recombinant into cloning vector vector into a host Puaron Eduption ine 3.Introduction of cloned DNA into host organism Chen Feng,Lecture of MicrobiologyChen Feng, Lecture of Microbiology 11.3 Essentials of Molecular Cloning Molecular cloning: isolation and incorporation of a piece of DNA into a vector so it can be replicated and manipulated Three main steps of gene cloning: 1. Isolation and fragmentation of source DNA 2. Insertion of DNA fragment into cloning vector 3. Introduction of cloned DNA into host organism