Enrichment and isolation methods Enrichment culture technique: a medium and a set of incubation conditions are used that are selective for the desired organism and are counterselective for the undesired organisms The Winogradsky column: for isolation of purple and green phototrophic bacteria and other anaerobes From enrichments to pure cultures: Steak plate and agar shake tube methodEnrichment and Isolation Methods Enrichment culture technique: a medium and a set of incubation conditions are used that are selective for the desired organism and are counterselective for the undesired organisms. The Winogradsky column: for isolation of purple and green phototrophic bacteria and other anaerobes. From enrichments to pure cultures: Steak plate and agar shake tube method