[实验步骤] 2.融合 加GKN溶液至0.5ml,混匀 Q正 置于39℃水浴5min 缓慢逐滴加0.25m1的50%PEG至悬液，边加边混匀 置于39℃7水浴15min 加入GKN溶液至4ml,混匀 置于39℃水浴15min 3000rpm,离心1min,弃上清 见 加入GKN溶液至4ml,混匀，3000rpm,离心1min This document is produced by trial version of Print2Flash.Visit www.print2flash.com for more informationThis document is produced by trial version of Print2Flash. Visit www.print2flash.com for more information