How big should your pinhole be? Width of point spread function at pinhole Airy disk diameter x magnification of lens 1 Airy unit =resolution of lens×magnification of lens×2 -100x 1.4 NA:resolution 220nm,so 1 Airy unit 44 um -40x/1.3 NA:resolution 235nm,so 1 Airy unit 19 um -20x/0.75 NA:resolution 407nm,so 1 Airy unit 16 um 10x/0.45 NA:resolution 678nm,so 1 Airy unit 14 umHow big should your pinhole be? • Width of point spread function at pinhole = Airy disk diameter × magnification of lens = 1 Airy unit = resolution of lens × magnification of lens × 2 – 100x / 1.4 NA: resolution = 220nm, so 1 Airy unit = 44  m – 40x / 1.3 NA: resolution = 235nm, so 1 Airy unit = 19  m – 20x / 0.75 NA: resolution = 407nm, so 1 Airy unit = 16  m – 10x / 0.45 NA: resolution = 678nm, so 1 Airy unit = 14  m