Question 4 Shown below is a diagram of a replication fork in a double-stranded(ds) DNA molecule found in a prokaryotic cell. Each DNA strand(a and B)serves as a template for polymerization by DNA polymerase, resulting in the formation of a newly synthesized"daughter"DNA strand strand a Replication fork moves in this direction strand B i)On which template strand(A or B)would there be continuous replication by DNA polymerase? What is this newly synthesized daughter strand called during dNA Strand a will be copied in a continuous fashion. The daughter strand will be the leading strand ii)On which template strand (A or B)would there be discontinous replication by dna polymerase? What is this newly synthesized daughter strand called during DNA replication Strand b will be copied in a discontinuous fashion. The daughter strand will be the lagging strand iii)Chemicals that inhibit the enzyme DNa ligase will primarily affect synthesis on one of the two template strands(A or B). Explain on which template strand (a or B) polymerization will be primarily affected and why this occurs Synthesis of the lagging strand using strand b as a template will be affected most by inhibition of DNA ligase. Without dNA ligase, the Okazaki fragment can not be joined together to form a contiguous length of dna b) There are inaccuracies in the dna molecule shown below 5′ A GTCCGAUGO3 5TCAGGCTATG 3 i)Name three things that are wrong in the above dNa seque 012Fal20037.012 Fall 2003 Question 4 Shown below is a diagram of a replication fork in a double-stranded (ds) DNA molecule found in a prokaryotic cell. Each DNA strand (A and B) serves as a template for polymerization by DNA polymerase, resulting in the formation of a newly synthesized "daughter" DNA strand. Replication fork moves in this direction 5’ 3’ 3’ 5’ strand A strand B a) i) On which template strand (A or B) would there be continuous replication by DNA polymerase? What is this newly synthesized daughter strand called during DNA replication? Strand A will be copied in a continuous fashion. The daughter strand will be the leading strand. ii) On which template strand (A or B) would there be discontinous replication by DNA polymerase? What is this newly synthesized daughter strand called during DNA replication? Strand B will be copied in a discontinuous fashion. The daughter strand will be the lagging strand. iii) Chemicals that inhibit the enzyme DNA ligase will primarily affect synthesis on one of the two template strands (A or B). Explain on which template strand (A or B) polymerization will be primarily affected and why this occurs. Synthesis of the lagging strand using strand B as a template will be affected most by inhibition of DNA ligase. Without DNA ligase, the Okazaki fragment can not be joined together to form a contiguous length of DNA. b) There are inaccuracies in the DNA molecule shown below.


1 5 10


5' A G T C C G A U G C 3' | | | | | | | | | |


5' T C A G G C T A T G 3' i) Name three things that are wrong in the above DNA sequence