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22.2 Nuclear splice junctions are interchangeable but are The vector cont ains two exons that are spliced together in the transcrip promotery 5' splice junction! 3 splice junction Figure 2.20 A special exon int ron exon splicing vector is used for exon trapping. If an A genomic fragment is inserted into the intron, any exon in the exon is present in the fragment will be found in the RNA Genomic fragment genomic fragment, its intron exon intron sequence will be Insert into recovered in the cloning site cytoplasmic RNA, but if the genomic fragment consists exon exon intron solely of an intron Transcrip splicing 消当Figure 2.20 A special splicing vector is used for exon trapping. If an exon is present in the genomic fragment, its sequence will be recovered in the cytoplasmic RNA, but if the genomic fragment consists solely of an intron, 22.2 Nuclear splice junctions are interchangeable but are read in pairs
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