10.7 Reaction rate and rate equation 10.7.2 Continuous flow method doi:10.1006/mbi2000.4124availableonlineathttp:/www.idealibrary.comonIdEalj.mOlBial.(2000)303,773-795 JMB 几 Multiple-step Kinetic Mechanism of DNA-independent ATP Binding and Hydrolysis by Escherichia coll Replicative Helicase DnaB Protein: Quantitative Analysis Using the Rapid Quench-flow Method Surendran Rajendran, Maria J. Jezewska and Wlodzimierz Bujalowski Difficulty:(1)a large amount of solution is required;(2) steady and exact motion ./ of the detector. 0.05 0.1 0.15 Time(Difficulty: (1) a large amount of solution is required; (2) steady and exact motion of the detector. 10.7 Reaction rate and rate equation 10.7.2 Continuous flow method