BEH. 462/3.962J Molecular Principles of Biomaterials Spring 2003 10 SPR Arrays External analysi ctIOn Optical method Cell- and tissue-based biosensors(Stenger 2001, Gross 1997) General concepts Why cell-based biosensors? o Known ultrasensitivity of cells: Olfactory neurons respond to single odorant molecules Retinal neurons triggered by single photons T cells triggered by single antigenic peptides(Irvine 2002) DICAra oN Calcium sigr P 风 Time relative to Ca* sgnal PE fjoreeceneo Potential for single PE nucnescenoe signal In TAPg ntertege molecule sensitvity 白 g Teel rogerian offorelgn pepNde shown at rght Positon如m Complex evaluation of 405067080 o ability to'integrate cellular or tissue response to compound ne et al.,2002] Detect functionality of compound in addition to its chemical presence e. tell the difference between a dead and live virus Cell-based biosensors are based on a primary transducer( the cell) and secondary transducer(device which converts cellular/biochemical response into a detectable signal) on Secondary transducer may be electrical or optical Detection of arbitrary targets on Transfect cells with receptors to introduce responsiveness of e.g. neuronal cells to a chosen compound Basis of electrical secondary transducers Electrically-excitable cells Example cell types Neurons Lecture 19-BiosensorsBEH.462/3.962J Molecular Principles of Biomaterials Spring 2003 SPR Arrays10 ƒ External analysis/detection ƒ Optical method Cell- and tissue-based biosensors (Stenger 2001, Gross 1997) General concepts ƒ Why cell-based biosensors? o Known ultrasensitivity of cells: ƒ Olfactory neurons respond to single odorant molecules ƒ Retinal neurons triggered by single photons ƒ T cells triggered by single antigenic peptides (Irvine 2002) o Ability to ‘integrate’ cellular or tissue response to compounds ƒ Detect functionality of compound in addition to its chemical presence • i.e. tell the difference between a dead and live virus ƒ Cell-based biosensors are based on a primary transducer (the cell) and secondary transducer (device which converts cellular/biochemical response into a detectable signal) o Secondary transducer may be electrical or optical ƒ Detection of arbitrary targets o Transfect cells with receptors to introduce responsiveness of e.g. neuronal cells to a chosen compound ƒ Basis of electrical secondary transducers o Electrically-excitable cells ƒ Example cell types • Neurons Lecture 19 – Biosensors