Figure 11.6 Transformant colonies growing on agar surface Replica-plate onto membrane filter Partially lyse cells;add Lyse bacteria and denature specific antibody;add agent DNA;add RNA or DNA to detect bound antibody in probe(radioactive);wash radiolabeled form out unbound radioactivity ⊙ 0 g o Autoradiograph to detect radioactivity X-ray film Positive- colonies (a) (b) 2012 Pearson Education,IncFigure 11.6 Transformant colonies growing on agar surface X-ray film Positive colonies Replica-plate onto membrane filter Partially lyse cells; add specific antibody; add agent to detect bound antibody in radiolabeled form Lyse bacteria and denature DNA; add RNA or DNA probe (radioactive); wash out unbound radioactivity Autoradiograph to detect radioactivity © 2012 Pearson Education, Inc