Characteristics of Plant Tissue Culture Techniques 1. Occur on a micro-scale 2. Environmental conditions optimized (nutrition, light, temperature). 3. All microbes(fungi, bacteria, viruses, and nematodes are excluded) 4. The normal pattern of plant development breaks down giving rise to callus, adventitious roots shoots and embryosCharacteristics of Plant Tissue Culture Techniques 1. Occur on a micro-scale. 2. Environmental conditions optimized (nutrition, light, temperature). 3. All microbes (fungi, bacteria, viruses, and nematodes are excluded). 4. The normal pattern of plant development breaks down giving rise to callus, adventitious roots, shoots, and embryos