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解释-2蛋白质如何结晶 (混乱→有序熵不利)? Temperature HANGING DROP Concentration PH value Protein Conc.x l/2 Precipitant Conc.x 1/2 饱和蒸气 足够 Vol.x 2 Purity Bvffer 压变化 Additives Precipitants 500uL REAGENT I-2uL PROTEIN Crystals of HSA-myristic acid complex were obtained with the hanging diffusion method in a 28%PEG 3350,50 mM phos phate buffer(pH 7.5),and 150 mM KCI.The crystals of OsrHSA were flash-frozen in liguid pitrogen and the Xrav diffraction data were collected at the synchrotron beamline to 2.05 A res- olution with the crystal maintained at the temperature of 100 K. https://www.bilibili.com/video/BV10E411d7Vx/?spm_id_from=autoN 高度有序排列(含水) ext&t=7.3解释-2 蛋白质如何结晶(混乱→有序熵不利)? 纯度高 足够浓 https://www.bilibili.com/video/BV1oE411d7Vx/?spm_id_from=autoN ext&t=7.3 饱和蒸气 压变化 高度有序排列(含水) 严禁复制
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