Conditions influencing the effectiveness of antimicrobial agent activity 1. Population size 2. Population composition. Spore, young/mature 3. Concentration or intensity of an antimicrobial agent 4. Duration of exposure 5. Temperature higher ·6. local environment eg. 1. Heat kill more readily at an acid ph 2. Organic matter protect microorganisms 3. Biofilm protectionConditions influencing the effectiveness of antimicrobial agent activity • 1. Population size. • 2. Population composition. Spore, young/mature • 3. Concentration or intensity of an antimicrobial agent. • 4. Duration of exposure. • 5. Temperature. higher • 6. local environment. eg. 1. Heat kill more readily at an acid pH. 2. Organic matter protect microorganisms. 3. Biofilm protection