Antimicrobials that bind to the 30s Ribosomal subunit Aminoglycosides (protein synthesis inhibitor -bactericidal) streptomycin, kanamycin, gentamicin, tobramycin amikacin netilmicin, neomycin · Modes of action Irreversibly bind to the 16S ribosomal rna and freeze the 30S initiation complex(30S-mRNA-tRNA) and prevents initiation of translation Induces misreading of the mRNA Destabilize microbial membranes Multiple modes of action -bactericidalAminoglycosides (protein synthesis inhibitor -bactericidal) streptomycin, kanamycin, gentamicin, tobramycin, amikacin, netilmicin, neomycin • Modes of action – Irreversibly bind to the 16S ribosomal RNA and freeze the 30S initiation complex (30S-mRNA-tRNA) and prevents initiation of translation. – Induces misreading of the mRNA – Destabilize microbial membranes – Multiple modes of action -bactericidal Antimicrobials that Bind to the 30S Ribosomal Subunit