Widal test Procedure 1. array forty test tubes into 4 rows, 10 tubes per row. Label the dilution ratio in every tube 2. Add 05ml saline into the first tube. and 0 5ml saline into other tubes in every row 3. Add the diluted serum 0 5ml( the dilution is 1: 5)to the first tube of first row, and beat upon three times. Then transfer 0 5ml to the second tube Repeat these one by one till the ninth tube, and then discard 0.5 ml from the ninth tube Only add 05ml saline in the tenth tube to compare 4. Do the same in the next 3 rows. Then the dilution ratio of every row in turn is 1: 10. 1: 20. 1: 40.1: 80.1: 160 till to 1: 2560. The tenth tube is comparative tube 88 Procedure 1.Array forty test tubes into 4 rows, 10 tubes per row. Label the dilution ratio in every tube. 2.Add 0.5ml saline into the first tube, and 0.5ml saline into other tubes, in every row. 3.Add the diluted serum 0.5ml(the dilution is 1:5) to the first tube of first row, and beat upon three times. Then transfer 0.5ml to the second tube. Repeat these one by one till the ninth tube, and then discard 0.5 ml from the ninth tube. Only add 0.5ml saline in the tenth tube to compare. 4.Do the same in the next 3 rows. Then the dilution ratio of every row in turn is 1:10, 1:20, 1:40, 1:80, 1:160 till to 1:2560. The tenth tube is comparative tube. Widal test