Plate Count After incubation,count colonies on plates that have 25- 250 colonies (CFUs) 1 ml 1 ml 1 ml 1 ml 1 ml Original 9 ml broth inoculum in each tube Dilutions 1:10 1:100 1:1000 1:10,000 1:100,000 ml ml ml 1 ml 1 ml Plating 1:10 1:100 1:1000 1:10,000 1:100,000 Calculation:Number of colonies on plate x reciprocal of dilution of sample nmber of bacteria/ml (For example,if 32 colonies are on a plate of /10,o00 dilution,then the count is 32 x 10,000 320,000/ml in sample.) Figure 6.15• After incubation, count colonies on plates that have 25- 250 colonies (CFUs) Plate Count Figure 6.15