(A)Initiation of strand synthesis DNA Sequencing Primer TT T5 ( B)A dideoxynucleotide Template DNA 当■_首 LLL T干T5 →P一 0-P-0一CH2 TTT 5 HH (C) Strand synthesis terminates when a ddNTP is added TT T DNA sequence dda GAATTGGCGGG dda GAATTGGCGG GAAT TGGCG TT T GAATTGGC dda GAATTGG GAATTG AATT GAAT y人 GAA TT A Adapted from Fig. 4.2 of "Genomes" by T A Brown, John Wiley Sons, NY, 1999DNA Sequencing Adapted from Fig. 4.2 of “Genomes” by T. A. Brown, John Wiley & Sons, NY, 1999