mRNA-target of miRNA m7G cap AAAAAACA) miRNA- directed RNA/siRNA cleavage m7G 3OH 5'p AAAAAA(A cap 3'end of cleaved mRNA 3'end of cleaved mRNA 5'P AAAAAACA) PARE: 5'RNA adapter ligation parallel analysis AAAAAA(A) of RNA ends 4一T)21 Reverse transcription 5 2nd strand synthesis(make cDNA) PARE/ GMUCT degradome sequencing Sonication Mme l Cut to capture 20-21 Sonicate and select~200-bp GMUCT: bp signature adapter lig ted fraction genome-wide mapping of uncapped and leaved Add 3'adapter,PCR amplify transcript Short-read sequencingmRNA targets of miRNA/siRNA  Analyses of mRNA targets of miRNA and mRNA degradation products by NGS methods. PARE: parallel analysis of RNA ends GMUCT: genome-wide mapping of uncapped and leaved transcript