Some synthetases are able to Adenylation proofread the incorrectly incorporated amino acids Proofreading: aminoncyl-adernylate is hydrolyzed tRNA binding amino acid C At the aminoacyl- AMP stage hanging with tRNA Proofreading aminoacyl-tRNA is hydrolyzed Wrong amino acid a At the aminoacyl- ERNA Stage COOH Correct amino acid AMP Correctly Aminoacyl-tRNA charged tRNAAt the aminoacyl￾AMP stage At the aminoacyl￾tRNA stage Correctly charged tRNA Some synthetases are able to proofread the incorrectly incorporated amino acids