BEIJING UNIVERSITY OF CHEMICAL TECHNOLOGY Derive the rate equation for the formation of the double helix and express the rate constant of the renaturation reaction in terms of the rate constants of the individual steps. We assume a pre-equilibrium(as the initial step is fast),and write ,implying that hel=KCACg C.CR The rate-determining step then gives v=C=kC=kKC,C=kC,C。k=k灯 d The equilibrium constant is the outcome of the two processes k A+B unstable helix. k Therere.wh C..-答 TEL:010-64434903BEIJING UNIVERSITY OF CHEMICAL TECHNOLOGY TEL:010-64434903 2 Derive the rate equation for the formation of the double helix and express the rate constant of the renaturation reaction in terms of the rate constants of the individual steps. We assume a pre-equilibrium (as the initial step is fast), and write A B unstablehelix C C C K = , implying that Cunstable helix=KCACB The rate-determining step then gives unstablehelix A B A B doublehelix k C k KC C kC C dt dC v = = 2 = 2 = [k=k2K] The equilibrium constant is the outcome of the two processes k1 A +B unstable helix. k2 ' 1 1 k k K = Therefore, with ACB v = kC , ' 1 1 2 k k k k =