实验原理 Monoclonal antibody Antigen Tumor Antibody- cells Immunization Isolate forming cells immune cells Fusion Hybridomas 66 Hybridomas screened Clonal for production of desired antibody Monoclonal expansion antibodies Antibody-producing hybridomas cloned Freeze Kohler,G.and Milstein,C.(1975)Continuous cultures of fused cells secreting antibody of predefined specificity.Nature 256,495-497 遵义医科大学免疫学教研室 DEPARTMENT OF IMMUNOLOGYAMPLIFICATION 遵义医科大学免疫学教研室 DEPARTMENT OF IMMUNOLOGY， ZMU 实验原理