EcoRI等产生的5'粘性未端 5.G-C-T-G-A-A-T-T-C-G-A-G.3' I IIIIIIIIIII 3.C-G-A-C-T-T-A-A-G-C-T-C.5' EcoRI37℃ 5.G-C-T-G-OH P-A-A-T-T-C-G-A-G.3' 1111 1111 3.C-G-A-C-T-T-A-A-9 ©H-G-C-T-C.5 退火4-7℃ OH P 5.G-C-T-G A-A-T-T-C-G-A-G.3' 111111111111 3.C-G-A-C-T-T-A-A G-C-T-C.5 P OH