INSIGHT INTRODUCTION NATURE Vol 447 24 May 2007 doi:10.1038/nature05913 Perceptions of epigenetics Adrian bird Geneticists study the gene; however, for epigeneticists there is no obvious 'epigene. Nevertheless, during the past year, more than 2, 500 articles, numerous scientific meetings and a new journal were devoted to the subject of epigenetics. It encompasses some of the most exciting contemporary biology and is portrayed by the popular press as a revolutionary new science an antidote to the idea that we are hard-wired by our genes. So what is epigenetics? There has always been a place in biology for words that have different to genetic determinism. A recent BBC television science programme meanings for different people. Epigenetics is an extreme case, because it hailed the advent of epigenetics as a profound shift in our understanding hasseveralmeaningswithindependentrootstoConradWaddingtonofinheritance(http://www.bbc.co.uk/sn/tvradio/programmes/horizon/ it was the study of epigenesis: that is, how genotypes give rise to pheno- ghostgenes. shtml). It summarized the implications of the emergent so ypes during development. By contrast, Arthur Riggs and colleagues ence as follows: At the heart of this new field is a simple but contentious defined epigenetics as"the study of mitotically and/or meiotically herit- idea-that genes have a memory. That the lives of your grandparents ble changes in gene function that cannot be explained by changes in -the air they breathed, the food they ate, even the things they saw-can DNA sequence": in other words, inheritance, but not as we know it. directly affect you, decades later, despite your never e y s. dbo.e These definitions differ markedly, although they are often conflated as things yourself. Is there any evidence for these heady though they refer to a single phenomenon. Waddingtons term encom- reliable is it? The answer to the first part of the question is yes. passes the activity of all developmental biologists who study how gene Sufa y during development causes the phenotype to emerge, but it Genes learning by experience? suffers from the disadvantage that developmental biologists themselves Several studies have reported evidence that links the environment rarely, if ever, use this word to describe their field. In this sense, the or ageing to long-lasting epigenetic effects on phenotype. One study usage is obsolete. The definition put forward by riggs and colleagues saving open what kinds of mechanism are at work. In this artide lpe o tells us what epigenetics is not(inheritance of mutational cha Box 1 Epigenetic paradigms ples of how epigenetic phenomena are studied and interpreted There are two classic epigenetic systems: the Polycomb and Trithorax (Polycomb/Trithorax) systems, and DNA methylation. The Polycomb and I propose a revised definition that embodies contemporary usage and Trithorax groups of proteins, which are named after mutants of the of the word. The molecular basis of heritable epigenetics has been studied in a transcription states, respectively, of developmentally important genes variety of organisms. The DNA methylation system and the Polycomb/ in the absence of these systems, the genes that specify the different Trithorax systems come closest to the ideal, because alterations in these systems are often inherited by subsequent generations of cells and some segments of the fruitfly are initially expressed correctly, but this pattern cannot be maintained. It can be inferred from this that the polycomb times organisms(Box 1). A classic case of what Robin Holliday named Trithorax systems stably'memorize'gene-expression patterns that mutation is the peloric variant of toadflax(Linaria) flowers(Fig. 1), ave been set up by other cellular mechanisms. There is evidence that first described by Linnaeus. In this variant, heritable silencing of the Polycomb-imposed silencing can even be transmitted between fruitfly gene Lcyc, which controls flower symmetry, is due not to a conventional generations at low frequency8Biochemical studies have enabled the mutation(that is, a mutation in the nucleotide sequence) but to the identification of components of the two key Polycomb-system protein stable transmission of DNA methylation at this locus from generation complexes and have established a close link with modification of the to generation. Although most variants arising in laboratory plants ar ine residue at position 27 of histone H3. The mechanism by which due to conventional mutations rather than epimutations of this kind, silencing is transmitted between cell generations remains obscure examples of transgenerational epigenetics are now well documented in the case of DNA methylation, biochemical information preceded plants(see page 418)and fungi. In animals, however, the transmission genetic understanding of the system. The methylated sequence of epigenetic traits between organismal generations has, so far, been in vertebrates is cG, which is paired with the same sequence on detectable only by using highly sensitive genetic assays. The m agoutilocus(also known as nonagouti), which affects coat colour, is the transiently methylated on only one of the two DNA strands(that is, best-studied example, being affected by the extent of DNA methylation are copied between cell generations by the DNA methyltransferase at an upstream transposon. Genetically identical parents whose agouti DNMTI, which completes hemimethylated but not unmethylated genes are in different epigenetic states tend to produce offspring with sites. In plants and fungi, the base 5-methylcytosine is also present in Despite the paucity of data from animal studies, this type of epigenetic non-symmetrical DNA sequences, so the mechanism of copying is less obvious DNA methylation is associated with stable gene silencing as caught the general imagination because, in principle, it is stable but example, on the inactive X chromosome), either through interference potentially affected by the environment. The possibility that acquired with transcription-factor binding or through the recruitment of marks'can be passed from parents to children has a deliciously repressors that specifically bind sites containing methylated CG lamarckian flavour that has proved difficult to resist as a potential antidot Wellcome Trust Centre for Cell Biology Edinburgh University The King s Buildings, Edinburgh EH9 3JR, UK. 396 @2007 Nature Publishing GroupThere has always been a place in biology for words that have different meanings for different people. Epigenetics is an extreme case, because it has several meanings with independent roots. To Conrad Waddington, it was the study of epigenesis: that is, how genotypes give rise to pheno￾types during development1 . By contrast, Arthur Riggs and colleagues defined epigenetics as “the study of mitotically and/or meiotically herit￾able changes in gene function that cannot be explained by changes in DNA sequence”2 : in other words, inheritance, but not as we know it. These definitions differ markedly, although they are often conflated as though they refer to a single phenomenon. Waddington’s term encom￾passes the activity of all developmental biologists who study how gene activity during development causes the phenotype to emerge, but it suffers from the disadvantage that developmental biologists themselves rarely, if ever, use this word to describe their field. In this sense, the usage is obsolete. The definition put forward by Riggs and colleagues tells us what epigenetics is not (inheritance of mutational changes), leaving open what kinds of mechanism are at work. In this article, I give examples of how epigenetic phenomena are studied and interpreted, and I propose a revised definition that embodies contemporary usage of the word. The molecular basis of heritable epigenetics has been studied in a variety of organisms.The DNA methylation system and the Polycomb/ Trithorax systems come closest to the ideal, because alterations in these systems are often inherited by subsequent generations of cells and some￾times organisms (Box 1). A classic case of what Robin Holliday named epimutation3 is the peloric variant of toadflax (Linaria) flowers (Fig. 1), first described by Linnaeus. In this variant, heritable silencing of the gene Lcyc, which controls flower symmetry, is due not to a conventional mutation (that is, a mutation in the nucleotide sequence) but to the stable transmission of DNA methylation at this locus from generation to generation4 . Although most variants arising in laboratory plants are due to conventional mutations rather than epimutations of this kind, examples of transgenerational epigenetics are now well documented in plants (see page 418) and fungi. In animals, however, the transmission of epigenetic traits between organismal generations has, so far, been detectable only by using highly sensitive genetic assays5 . The mouse agouti locus (also known as nonagouti), which affects coat colour, is the best-studied example, being affected by the extent of DNA methylation at an upstream transposon. Genetically identical parents whose agouti genes are in different epigenetic states tend to produce offspring with different coat colours, although the effect is variable. Despite the paucity of data from animal studies, this type of epigenetics has caught the general imagination because, in principle, it is stable but potentially affected by the environment. The possibility that acquired ‘marks’ can be passed from parents to children has a deliciously lamarckian flavour that has proved difficult to resist as a potential antidote to genetic determinism. A recent BBC television science programme hailed the advent of epigenetics as a profound shift in our understanding of inheritance (http://www.bbc.co.uk/sn/tvradio/programmes/horizon/ ghostgenes.shtml). It summarized the implications of the emergent sci￾ence as follows: “At the heart of this new field is a simple but contentious idea — that genes have a ‘memory’. That the lives of your grandparents — the air they breathed, the food they ate, even the things they saw — can directly affect you, decades later, despite your never experiencing these things yourself.” Is there any evidence for these heady claims, and how reliable is it? The answer to the first part of the question is yes. Genes learning by experience? Several studies have reported evidence that links the environment or ageing to long-lasting epigenetic effects on phenotype. One study Perceptions of epigenetics Adrian Bird1 Geneticists study the gene; however, for epigeneticists, there is no obvious ‘epigene’. Nevertheless, during the past year, more than 2,500 articles, numerous scientific meetings and a new journal were devoted to the subject of epigenetics. It encompasses some of the most exciting contemporary biology and is portrayed by the popular press as a revolutionary new science — an antidote to the idea that we are hard-wired by our genes. So what is epigenetics? 1 Wellcome Trust Centre for Cell Biology, Edinburgh University, The King’s Buildings, Edinburgh EH9 3JR, UK. There are two classic epigenetic systems: the Polycomb and Trithorax (Polycomb/Trithorax) systems, and DNA methylation. The Polycomb and Trithorax groups of proteins, which are named after mutants of the fruitfly Drosophila melanogaster, work to maintain repressed or active transcription states, respectively, of developmentally important genes. In the absence of these systems, the genes that specify the different segments of the fruitfly are initially expressed correctly, but this pattern cannot be maintained. It can be inferred from this that the Polycomb/ Trithorax systems stably ’memorize’ gene-expression patterns that have been set up by other cellular mechanisms. There is evidence that Polycomb-imposed silencing can even be transmitted between fruitfly generations at low frequency18. Biochemical studies have enabled the identification of components of the two key Polycomb-system protein complexes and have established a close link with modification of the lysine residue at position 27 of histone H3. The mechanism by which silencing is transmitted between cell generations remains obscure. In the case of DNA methylation, biochemical information preceded genetic understanding of the system. The methylated sequence in vertebrates is CG, which is paired with the same sequence on the opposite DNA strand. This symmetry means that sites are transiently methylated on only one of the two DNA strands (that is, hemimethylated) after DNA replication. CG methylation patterns are copied between cell generations by the DNA methyltransferase DNMT1, which ‘completes’ hemimethylated but not unmethylated sites. In plants and fungi, the base 5-methylcytosine is also present in non-symmetrical DNA sequences, so the mechanism of copying is less obvious. DNA methylation is associated with stable gene silencing (for example, on the inactive X chromosome), either through interference with transcription-factor binding or through the recruitment of repressors that specifically bind sites containing methylated CG. Box 1 | Epigenetic paradigms 396 INSIGHT INTRODUCTION NATURE|Vol 447|24 May 2007|doi:10.1038/nature05913 ￾