7.03 Lecture 28-30 11/17/03,11/19/03,11/21/03 The methods of genetic analysis that you have been learning are applicable to mammals-even to humans.However,we need to combine these genetic principles with an understanding of the physical realities of the human genome.To genetics we will add genomics. Eukaryotic Genes and Genomes genome DNA content of a complete haploid set of chromosomes DNA content of a gamete (sperm or egg) DNA year Species Chromosomes cM content/ genes/ sequence haploid(Mb) completed haploid E.coli 1 N/A 5 1997 4,200 S.cerevisiae 16 4000 12 1997 5,800 C.elegans 6 300 100 1998 19,000 D.melanogaster 4 280 180 2000 14,000 M.musculus 20 1700 3000 2002 draft 30,0002 2005 finished? H.sapiens 23 3300 3000 2001 draft 30,0002 2003 finished Note: CM centi Morgan 1%recombination Mb megabase=1 million base-pairs of DNA Kb kilobase 1 thousand base-pairs of DNA 27.03 Lecture 28-30 11/17/03, 11/19/03, 11/21/03 2 The methods of genetic analysis that you have been learning are applicable to mammals — even to humans. However, we need to combine these genetic principles with an understanding of the physical realities of the human genome. To genetics we will add genomics. Eukaryotic Genes and Genomes = DNA content of a gamete (sperm or egg) genome = DNA content of a complete haploid set of chromosomes H. sapiens M. musculus D. melanogaster C. elegans S. cerevisiae E. coli genes/ haploid year sequence completed DNA content/ haploid(Mb) Species Chromosomes cM 1 16 6 4 20 23 N/A 4000 300 280 1700 3300 5 12 100 180 3000 3000 1997 1997 1998 2000 4,200 5,800 19,000 14,000 30,000? 30,000? Mb = megabase = 1 million base-pairs of DNA Kb = kilobase = 1 thousand base-pairs of DNA Note: cM = centi Morgan = 1% recombination 2002 draft 2001 draft 2005 finished? 2003 finished