Principles Adsorption column chromatography and Thin-layer chromatography (TLC): separation is achieved by using the different adsorption and desorption capacities of the components in the mixture in the insoluble two phases (i.e. the mobile phase and the stationary phase) 2. The mixture of ferrocene and acetylferrocene is mainly isolated by utilizing the difference in adsorption capacity of silica gel 3 FePrinciples 1. Adsorption column chromatography and Thin-layer chromatography (TLC) : separation is achieved by using the different adsorption and desorption capacities of the components in the mixture in the insoluble two phases (i.e. the mobile phase and the stationary phase) 2. The mixture of ferrocene and acetylferrocene is mainly isolated by utilizing the difference in adsorption capacity of silica gel