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Using plasmid vectors (a)Human DNA Plasmid vectors EcoRI site EcoRI site Q Origin of replication a are cut with Ecai ectors DNA and plasmid v Gene for ampicillin resistance Cleaved fragments and vectors are combined in the presence of ligase. Ligase (b)Recombinant plasmids are added to a population of E. coli cells Host chromosome 9os E coli plated onto medium containing ampicillin Cells containing recombinant plasmids are able to grow
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