转录起始需要RNA聚合酶全酶 Polymerase bound nonspecifically to DNA 0N00N0NN0000N0N000000000000A00A0O0NA0000N0000 35 -10+1 Promoter L Specific binding of a to -35 and-10 promoter sequences Closed-promoter complex ailiii 8N0N0N00N000NN0N00N00N0100000NY -10+ Unwinding of DNA around the initiation site Open-promoter complex N0000000000N0000N0N2009X00N0N00N0000N0N00000000N000N Initiation of transcription转录起始需要RNA聚合酶全酶