Pulsed- field gel electrophoresis(脉冲 电泳) (1)The electric field is applied in pulses that are oriented orthogonally(直角地) to each other (2) Separate DNA molecules according to their molecule weight, as well as to their shape and topological properties (3) Can effectively separate dna molecules over 30-50 kb and up to several Mb in length 1313 (1)The electric field is applied in pulses that are oriented orthogonally (直角地) to each other. (2)Separate DNA molecules according to their molecule weight, as well as to their shape and topological properties. (3)Can effectively separate DNA molecules over 30-50 kb and up to several Mb in length. Pulsed-field gel electrophoresis (脉冲 电泳)