cDNA-microarrays high-density oligonucleotide arrays mRNA reference sequence uoneredaId cDNA collection HHH田 perfect match mismatch probe set insert amplification by PCR vector specific prmers gene specfic primers in situ synthesis aqoId by photolithography printing coupling denaturing ratio Cy5/Cy3 ratio array 1/array 2 staining fragmented hybridization hybridization mixing biotin labeled uoneredaId 1o3me cRNA Cy3 Cy5 (generated using T7 promoter) Cy3 or Cy5 in vitro transcription labeled cDNA double-stranded cDNA modified oligodT cDNA synthesis CDNA synthesis (generated using T7 primer) total RNA total RNA cells/tissue cells/tissue