离子交换层析( ion-exchange chromatography) (b) lon-exchange chromatography Negatively charged protein yh Positively charged Elute negatively . o protein charged protein Collect with salt solution no sample positively (NaCl)00 Q on charged column proteins Nat C/ 阴离 父 换树 脂颗 粒(+) Positively charged gel bead 4321离子交换层析（ion-exchange chromatography） 阴离 子交 换树 脂颗 粒(＋)