Isolation of pure cultures Pure culture: a population of cells arising from a single cell to characterize an individual species. They are required for the careful study of an individual microbial species. Single colony How to get Dilute-pour/spread plate technique dilute→>pour/ spread→> isolated colonies 2. Streak plates: inoculating loopIsolation of pure cultures • Pure culture: a population of cells arising from a single cell, to characterize an individual species. They are required for the careful study of an individual microbial species. • Single colony • How to get 1. Dilute-pour/spread plate technique: dilute→pour/spread →isolated colonies 2. Streak plates: inoculating loop